For improved l-serine production, an l-serine dehydratase-defective mutant of Sarcina albida IAM 1012 was obtained. In the mutant, the activities of the enzymes responsible for l-serine production were as high as those in the parent strain, and, at a low glycine concentration, the mutant accumulated l-serine more efficiently than the parent. Under optimum conditions, 21 mg of l-serine per ml ac...

Abnormal N-methyl-d-aspartate receptor (NMDAR) function has been implicated in the pathophysiology of schizophrenia. d-serine is an important NMDAR modulator, and to elucidate the role of the d-serine synthesis enzyme serine racemase (Srr) in schizophrenia, we identified and characterized mice with an ENU-induced mutation that results in a complete loss of Srr activity and dramatically reduced ...

Non-enzymatic deamination of serine and cysteine is catalyzed by pyridoxal and certain metal salts at 100” (2). This finding suggested that pyridoxal phosphate might be involved in the enzymatic deamination of these amino acids. Vitamin B, has already been implicated in the desulfhydration of cysteine by rat liver (3) and of cysteine and homocysteine by bacteria (4). Several similarities of cys...

The human immunodeficiency virus type 1 matrix (MA) protein is phosphorylated during virion maturation on its C-terminal tyrosine and on several serine residues. Whereas MA tyrosine phosphorylation facilitates viral nuclear import, the significance of MA serine phosphorylation remains unclear. Here, we report that MA serine but not tyrosine phosphorylation is strongly enhanced by Nef. Mutations...

N-methyl-D-aspartate (NMDA) receptors play critical roles in synaptic transmission and plasticity. Activation of NMDA receptors by synaptically released L-glutamate also requires occupancy of co-agonist binding sites in the tetrameric receptor by either glycine or D-serine. Although D-serine appears to be the predominant co-agonist at synaptic NMDA receptors, the transport mechanisms involved i...

Abstract Many tumour cells show dependence on exogenous serine and dietary glycine starvation can inhibit the growth of these cancers extend survival in mice. However, numerous mechanisms promote resistance to this therapeutic approach, including enhanced expression de novo synthesis pathway (SSP) enzymes or activation oncogenes that drive synthesis. Here we inhibition PHGDH, first step SSP, co...

Among aging-induced impairments, those affecting cognitive functions certainly represent one the most major challenge to face improve elderly quality of life. In last decades, our knowledge on changes in morphology and function neuronal networks associated with normal pathological brain aging has rapidly progressed, initiating development different pharmacological behavioural strategies allevia...

Antibiotic acyldepsipeptide (ADEP) targets the bacterial ClpP serine protease and can inhibit growth of numerous species by activating/dysregulating activity within cell. The spirochete Leptospira interrogans harbors two isoforms (LepClpP1 LepClpP2). Supplementation ADEP in medium resulted inhibition growth. mediated activation LepClpP mixture was dependent on time allowed for self-assembly Lep...

D-serine, an endogenous co-agonist of NMDA receptors in vertebrate retina, may modulate glutamate sensitivity of retinal neurons. This study determined at the functional and molecular level the transport process responsible for D-serine in retinal Müller cells. RT-PCR and immunoblotting showed that serine racemase (SR), the synthesizing enzyme for D-serine, is expressed in the rMC-1 Müller cell...