Septins are highly conserved filamentous proteins first characterized in budding yeast and subsequently identified in must eukaryotes. Septins can bind and hydrolyze GTP, which is intrinsically related to their formation of septin hexamers and functional protein interactions. The human septin family is composed of 14 loci, SEPT1-SEPT14, which encode dozens of different septin proteins. Their ce...

MARK/PAR-1 protein kinases play important roles in cell polarization in animals. Kin1 and Kin2 are a pair of MARK/PAR-1 orthologs in the budding yeast Saccharomyces cerevisiae. They participate in the regulation of secretion and ER stress response. However, neither the subcellular localization of these two kinases nor whether they may have other cellular functions is clear. Here, we show that K...

We investigate certain properties of the Wheeler-DeWitt metric (for constant lapse) in canonical General Realtivity associated with its non-definite nature.

ART 1102 Methodologies in the History and Theory of Art (3 units) Introduction to a variety of theoretical, critical, and historical methods used in art history and art criticism. Preparation in recognizing and utilizing particular methodologies in scholarly research, professional work, and writing. Examination of primary and secondary theoretical texts from a range of historical eras to judge ...

ART 1102 Methodologies in the History and Theory of Art (3 units) Introduction to a variety of theoretical, critical, and historical methods used in art history and art criticism. Preparation in recognizing and utilizing particular methodologies in scholarly research, professional work, and writing. Examination of primary and secondary theoretical texts from a range of historical eras to judge ...

A high-throughput strategy for testing gene function would accelerate progress in our understanding of disease pathogenesis for the dimorphic fungus Blastomyces dermatitidis, whose genome is being completed. We developed a green fluorescent protein (GFP) sentinel system of gene silencing to rapidly study genes of unknown function. Using Gateway technology to efficiently generate RNA interferenc...

BACKGROUND Septins are members of a conserved family of GTPases found in organisms as diverse as budding yeast and mammals. In budding yeast, septins form hetero-oligomeric filaments that lie adjacent to the membrane at the mother-bud neck, whereas in mammals, they concentrate at the cleavage furrow of mitotic cells; in both cases, septins provide a required function for cytokinesis. What direc...

Septins are a conserved group of GTP-binding proteins that form hetero-oligomeric complexes which assemble into filaments. These are essential for septin function, including their role in cytokinesis, cell division, exocytosis and membrane trafficking. Septin 2 (SEPT2) is a member of the septin family and has been associated with neurofibrillary tangles and other pathological features of senile...