Because of controversies in the literature on nitric oxide (NO) production by plants, NO detection by the frequently used diaminofluorescein (DAF-2 and DAF-2DA) and by chemiluminescence were compared using the following systems of increasing complexity: (i) dissolved NO gas; (ii) the NO donor sodium nitroprusside (SNP); (iii) purified nitrate reductase (NR); and (iv) tobacco cell suspensions. L...

Plasma from hemodialysis patients evoked weak photon emissions (chemiluminescence) in a characteristic emission spectrum with a peak at 430 nm, attributed to attack by hydroxyl radicals generated from the iron-catalyzed breakdown of hydrogen peroxide (Fenton reaction), whereas plasma from normal healthy subjects showed a rather weak red chemiluminescence peak at around 680 nm, similar to that r...

dried aceton-chloroform extract of aerial parts of euphorbia spinidens bornm. ex prokh. endemic to iran, yielded two new triterpenoids, lup-20(29)-ene-33, 28 diol commonly known as betulin and (3β,23e)-cycloarta-23-ene-3,25-diol. the structures of the isolated compounds were elucidated by 13 c- and 1 h-nmr as well as 2d-nmr, ir and by the aid of mass fragmentation pattern. in phagocyte chemilum...

Replacing the peroxide bond of dioxetanone prevents chemiluminescence by making its thermolysis energetically unfavorable and without a singlet chemiexcitation pathway.

Surface plasmon spectroscopy (SPS) is commonly implemented as an analytical tool to characterize thin kinetic processes and binding reactions at interfaces.1 Due to the limited sensitivity of SPS for the detection of dilute analytes of low molecular mass, surface plasmon coupled fluorescence (SPCF) or, alternatively, surface plasmon coupled emission (SPCE) has been proposed as an alternative me...

Journal of Organic Chemistry is published by the American Chemical Society. 1155 Sixteenth Street N.W., Washington, DC 20036 Electrogenerated chemiluminescence. XIX. Preparation and chemiluminescence of 5,12-dibromo-5,12-dihydro-5,6,11,12-tetraphenylnaphthacene Csaba P. Keszthelyi, and Allen J. Bard J. Org. Chem., 1974, 39 (19), 2936-2937• DOI: 10.1021/jo00933a029 • Publication Date (Web): 01 M...

The chemiluminescence of fluorescein derivatives is greatly enhanced when they are conjugated to proteins. We exploited this property in devising a chemiluminescence immunoassay for rubella antibodies. In this sequential "sandwich"-type assay, tubes coated with rubella antigen are used for antibody separation and goat antibody to human IgG conjugated with fluorescein isothiocyanate is used for ...

It has been suggested that oxygen-derived free radicals may contribute to the myocardial injury associated with ischemia and reperfusion. As the presence of enhanced free radical generation is a prerequisite for such damage, several techniques have been used to provide evidence of increased oxygen free radical production during reperfusion; however, all such techniques have substantial limitati...