Clear native electrophoresis and blue native electrophoresis are microscale techniques for the isolation of membrane protein complexes. The Coomassie Blue G-250 dye, used in blue native electrophoresis, interferes with in-gel fluorescence detection and in-gel catalytic activity assays. This problem can be overcome by omitting the dye in clear native electrophoresis. However, clear native electr...

Aim: To compare the results of DNA amplification by the polymerase chain reaction (PCR) with immunofluorescence staining for detecting Pneumocystis carin'i in bronchoalveolar lavage specimens taken from symptomatic HIV seropositive patients with suspected P carinii pneumonia (PCP). Methods: Bronchoalveolar lavage specimens were obtained from 28 symptomatic HIV seropositive patients. Specimens w...

AIM To compare the results of DNA amplification by the polymerase chain reaction (PCR) with immunofluorescence staining for detecting Pneumocystis carinii in bronchoalveolar lavage specimens taken from symptomatic HIV seropositive patients with suspected P carinii pneumonia (PCP). METHODS Bronchoalveolar lavage specimens were obtained from 28 symptomatic HIV seropositive patients. Specimens w...

BACKGROUND Electrophoretic separation of serum and urine proteins has played a central role in diagnosing and monitoring plasma cell disorders. Despite limitations in resolution and analytical sensitivity, plus the necessity for adjunct methods, protein gel electrophoresis and immunofixation electrophoresis (IFE) remain front-line tests. METHODS We developed a MALDI mass spectrometry-based as...

BACKGROUND In this study, two-dimensional gel electrophoresis (2-DE) method was applied to determine and compare the protein spots expressed in the two field isolates of Leishmania major and recovered from the patients who were clinically sensitive and resistant to Glucantime® treatment. METHODS Leishmania parasites were isolated from the cutaneous lesions of two CL infected patients in Shira...

Background: The numerous methods and techniques employed in proteomics investigations generate vast quantities of heterogeneous data, both results and provenance. A standard representation of proteomics data and techniques is urgently required in order to document a proteomics investigation. A standard representation will facilitate data exchange, verification and further analysis, and pr...

Microsatellite markers are employed for genotyping of Basmati varieties and assaying purity of market samples. However, employment of diverse electrophoresis techniques across laboratories has resulted in inconsistent allele sizes, creating doubts about the suitability of the assay. This study evaluated agarose gel electrophoresis, slab gel electrophoresis, and capillary electrophoresis techniq...

Introduction. The main biological raw materials for the production of immunobiological preparations identification Bacillus anthracis are its specific antigens, protective antigen and EA1 protein.
 Purpose. To determine antigenic identity immunodominant proteins different genovariants B. isolated by gel chromatography electrophoresis.
 Materials methods. Culture filtrates isogenic var...