Aim: The present experiments were conducted (a) to evaluate the semen attributes of older Rhode Island Red (RIR) roosters and the inter-trait relationships, (b) to test sperm washing and storage duration suitable for gene transfer experiments. Materials and Methods: The semen characteristics of older RIR roosters were studied, and Pearson correlation analysis was done to demonstrate the inter-t...

Flow cytometry is considered the only reliable method for the separation of X and Y chromosome bearing spermatozoa in equines. The MoFlo SX DP sorter is highly efficient, allowing the production of foals of the desired sex. However, to achieve acceptable pregnancy rates the currently used protocol requires working with fresh semen obtained close to, or at, the sorting facility. An alternative p...

With the recent acceptance of frozen semen by the American Quarter Horse Association and the American Paint Horse Association, the overall interest and use of this breeding method has increased significantly in recent years. Proper handling and maintenance of semen frozen and stored in liquid nitrogen is critical to the success of artificial insemination with frozen semen. International distrib...

The aim of this review was to summarise recent knowledge of the physiology of semen production and management of rabbit bucks. As in other mammals, the spermatogenesis is affected by many environmental factors (temperature, light, age of animals, feeding strategies, health status) as well as by semen handling (dilution, temperature, and condition of storage). Optimal conditions of rearing rabbi...

The objective of this study was to evaluate the addition of IGF-I to pig insemination doses stored at 15°C, in conjunction with the addition of different amounts of vitamin E (α-tocopherol). Semen samples (n = 12) from four boars were treated by the addition of different concentrations of vitamin E, ranging up to 400 μg/ml. Immediately after processing and after the doses had been stored at 15°...

Because microfloral content of stallion semen tends to be high, and strains may be resistant to commonly used antibiotics, amikacin was tested with stallion semen and compared with bull semen. Nine ejaculates to stallion semen were incubated at 37 C in egg yolk-tris extender for 0, 2, 4, 6, 8 and 10 h in the presence of amikacin concentrations of 0, 50, 100, 250, 500, 1,000 and 10,000 microgram...