The study was conducted to determine the effect of 7-dehydrocholesterol loaded cyclodextrin (7-DHCLC) and cholesterol-loaded cyclodextrin (CLC) on changes of bull sperm motility during short term storage (+4°C). Collected ejaculates were pooled and divided into 7 groups, as following one control (C); three 7-DHCLC and the other three CLC concentrations. Diluted semen samples were transferred an...

The objective of this study was to specify the effects of centrifugation and different surface of soybean lecithin-based extender on post-thaw Sperm quality of Ghezel ram semen. Semen samples were gathered from 5 mature Ghezel ram two/week for 3 weeks. After initial evaluation, the approved semen samples were pooled together and split into equal parts in Falcon tubes. Three samples were combine...

The primary cause of sperm quality decline during the freeze-thaw pathway is peroxidation hazard caused by reactive oxygen species produced biological molecules sperm. Ascorbic acid (Vitamin C) and lycopene are two potent antioxidants that operate to prevent oxidation processes. This study aimed analyse effects ascorbic on motility, viability, abnormality plasma membrane integrity post-thawed S...

Background: This study was carried out to evaluate the protective effects of a cell permeable antioxidant, Manganese (III) meso-tetrakis (N-ethylpyridinium-2-yl) porphyrin chloride (MnTE), alone or in combination with different concentration of Catalase on motility of frozenthawed goat semen. Materials and Methods: Fresh semen ejaculates were collected with artificial vagina from 3 goats, mixed...

The aim of this study was to investigate the effect of PAF supplementation in semen extender on ATP content in cryopreserved bull spermatozoa used for artificial insemination at different time intervals. Cryopreserved semen was treated with different concentrations of PAF: 1×10-5M, 1×10-6M, 1×10-7M, 1×10-8M and 1×10-9M at 37°C. In the present work we showed that content of ATP in cryopreserved ...

Achromobacter xylosoxidans, a Pseudomonaslike bacterium, contaminated a boar stud laboratory’s water distillation and delivery system that was used for extension of raw semen for artificial insemination. In sow herds that used the contaminated semen, conception rates decreased and vulvar discharges, culled sows, and nonproductive sow days increased. Thorough diagnostic testing revealed that A x...

Sperm vitrification has been recently developed, but fertility trials have not performed yet in equine species. In this study, a new warming technique for vitrified donkey semen was developed and the uterine inflammatory response were compared to conventional freezing. Experiment 1, sperm straws warmed 3 ml of extender or water bath at: 37 °C/30 s; 43 °C/10 60 °C/5 s. motility, plasma acrosome ...

Supplementation of semen extender with caffeine and CaCl(2) for artificial insemination (AI) of fresh spermatozoa has been demonstrated to reduce recruitment of uterine polymorphonuclear leukocytes (PMNs) and the activity of phagocytosis. Here, we determined if addition of caffeine and CaCl(2) to semen extender improves the fertility of frozen-thawed boar semen. In experiment 1, gilts were cerv...

The aim of the present study was to evaluate cryoprotective effect trehalose on stallion semen quality like progressive sperm motility, viability, acrosome integrity, membrane integrity. A total 42 ejaculates from six Marwari (seven ejaculate each) were used. Immediately after gross evaluation, filtered into a pre warm, graduated measuring bottle get gel free and it diluted with primary extende...

It is known that livestock animal semen very sensitive to cold shock during freezing processes, and this sensitivity directly affects post-thaw progressive motility, mitochondrial membrane potential, sperm nuclear DNA integrity in vitro spermatological parameters such as plasma acrosome integrity, fertility. In addition, with the sudden decrease total antioxidant level of after thawing, cells a...