The aim of this study was to determine the influence of time interval between semen collection and artificial insemination (AI) and the number of spermatozoa in AI doses, on the AI success rate in Tunisian Arabian mares. A total of 79 AI with fresh semen was used on 56 mares (age 4-9 years, n = 34; age 10-20 years, n = 22). The AI success rate was defined as the number of pregnant mares divided...

Purpose: To evaluate the drop-to-drop and pellet-to-pellet repeatability and stability of frozen sperm pellets. Methods: Ten pellets were thawed per batch (low and normal concentration) and evaluated by two investigators to establish a quality control chart. Then low and normal concentration pellets were thawed and evaluated daily for 10 days by both investigators. The values for both investiga...

Abstract Study question Can comparable results be obtained for sperm concentrations determinations in differing clinical settings when using an AI driven analyser, a cytometer, and WHO manual counts? Summary answer Outcomes suggest that can utilising the counting techniques. What is known already Despite robust methods described by WHO, protocol variations exist, creating lack of reproducible c...

Storage of ram semen in liquid and frozen state, the diluents used for both methods, processing, cooling, freezing and thawing of semen are reviewed. Factors influencing the fertility of stored semen and methods used for improvement are discussed, and fertility results of long-term frozen stored ram semen are also given.

Objective To prospectively monitor Zika viral loads in semen from Belgian travellers with confirmed Zika virus infection, who returned from the Americas during the 2016 Zika virus epidemic. Methods We recruited symptomatic travellers consulting our clinic and we confirmed infection with either reverse-transcriptase (RT) polymerase chain reaction (PCR) assay or virus neutralization test. The p...

Some persistent organic pollutants (POPs) have been found in human semen but until this point it was unclear whether polybrominated diphenyl ethers (PBDEs) could be detected in human semen. In this study, PBDEs were found for the first time in human semen samples (n=101) from Taizhou, China. The concentrations of total PBDEs (∑PBDEs) varied from 15.8 to 86.8 pg/g ww (median=31.3 pg/g ww) and 53...

The mobility of pooled turkey sperm following various storage regimens was assessed by objectively measuring the ability of sperm to penetrate a 2% Accudenz [5-(N-2,3-dihydroxypropylacetamido)-2,4,6-tri-iodo-N,N'-bis(2,3-dihydroxypropyl)isophthalamide] solution at 41 C. When semen was diluted with Beltsville poultry semen extender and stored at 5 C with agitation at 150 rpm, sperm mobility decl...

The aim of the present study was to investigate the effect of in vitro supplementation of selenium on fresh and frozen spermatozoa quality of buffalo (Bubalus bubalis) bulls. Five healthy buffalo bulls (5 ejaculates from each bull) were used. Each ejaculate was diluted at 37 ˚C with tris-based extender containing 0 (control), 0.5, 1, 2, 4 and 8 µg mL(-1) sodium selenite and the sperm motility a...