The unicellular green alga Chlamydomonas reinhardtii is a model organism that provides an opportunity to understand the evolution and functional biology of the lineage that includes the land plants, as well as aspects of the fundamental core biology conserved throughout the eukaryotic phylogeny. Although many tools are available to facilitate genetic, molecular biological, biochemical, and cell...

The systematic sequencing of the yeast S.cerevisiae genome has revealed a profusion of Open Reading Frames (ORFs). Although some of them have been previously studied, a large majority represents new genes (1, 2). Deletion of these ORFs is a convenient tool for their functional analysis, Here we describe a new approach for generating null alleles of a gene. Usually, a gene inactivation requires ...