Six single- and multiple-site variants of Candida tenuis xylose reductase that were engineered to have side chain replacements in the coenzyme 2'-phosphate binding pocket were tested for NADPH versus NADH selectivity (R(sel)) in the presence of physiological reactant concentrations. The experimental R(sel) values agreed well with predictions from a kinetic mechanism describing mixed alternative...

Muxuan Pan 1, Kaiwen Zhang 1, Ye-Hwa Chen 2 and Jinquan Huang 1,* 1 College of Energy and Power Engineering, Nanjing University of Aeronautics and Astronautics, Nanjing 210016, Jiangsu, China; [email protected] (M.P.); [email protected] (K.Z.) 2 The George W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA 30324, USA; [email protected] ...

*Simona Luatti,1 *Fausto Castagnetti,1 Giulia Marzocchi,1 Carmen Baldazzi,1 Gabriele Gugliotta,1 Ilaria Iacobucci,1 Giorgina Specchia,2 Lucia Zanatta,3 Giovanna Rege-Cambrin,4 Marco Mancini,5 Elisabetta Abruzzese,6 Alfonso Zaccaria,7 Maria Grazia Grimoldi,8 Alessandro Gozzetti,9 Gaia Ameli,1 Maria Adele Capucci,10 Giandomenico Palka,11 Paolo Bernasconi,12 Francesca Palandri,1 Fabrizio Pane,13 G...