Objective HER-2/neu gene is overexpressed in diverse human cancers and studies suggest a role of its product – p185 protein – in tumor progression by specifically promoting the invasive capacity of tumor cells. Our aim was to evaluate HER-2/neu content in resectable gastric cancer in this geographical region and assess the relationship between p185 expression and clinicopathologic tumor paramet...

In recent years, the distinction between idiopathic follicular mucinosis (FM) and lymphoma-associated follicular mucinosis (LAFM) has been made through assessment of T-cell receptor gene rearrangement, flow cytometry, and immunohistochemistry. These methods, among others, have mostly identified monoclonality as a defining characteristic of LAFM; however, this finding cannot be considered conclu...

Abstract Vascular anomalies grouped into vascular tumors (hemangioma) (HI) and malformation (VM) are benign lesions that difficult to distinguish from one another clinically often confused with each other at histopathology. This confusing terminology leads improper diagnosis, illogical treatment, misdirected research. study aimed GLUT 1, S-100, nerve bundle differentiate hemangioma malformation...

CONTEXT The increasing demand for immunohistochemistry for clinical diagnostics, in combination with an ongoing shortage of staff in the histology laboratory, has brought about a need for automation in immunohistochemistry. The current automated staining platforms vary significantly in their design and capabilities. OBJECTIVE To review how technology has been applied to automating the process...

Being a significant health problem that affects patients in various age groups, breast cancer has been extensively studied to date. Recently, molecular breast cancer classification has advanced significantly with the availability of genomic profiling technologies. Proteomic technologies have also advanced from traditional protein assays including enzyme-linked immunosorbent assay, immunoblottin...

Non-Hodgkin's lymphoma (NHL) consists of a heterogeneous group of disorders which have been difficult to study by epidemiolÃ3gica!means in the past. However, recent advances in knowledge of the biology of NHL and improvements in its classification will greatly improve the quality of epidemiolÃ3gica!studies in the future. Use of the Working Formulation and the current International Classificatio...

Non-Hodgkin's lymphoma (NHL) consists of a heterogeneous group of disorders which have been difficult to study by epidemiological means in the past. However, recent advances in knowledge of the biology of NHL and improvements in its classification will greatly improve the quality of epidemiological studies in the future. Use of the Working Formulation and the current International Classificatio...

Histiocytic tumors represent a spectrum of neoplastic and immune-mediated diseases in dogs and cats. The treatments and prognoses for each of these diseases vary considerably. Until recently, much confusion surrounded the diagnosis and classification of histiocytic tumors, making it challenging to formulate consistent treatment recommendations for each tumor type. During the past decade, advanc...

Immunophenotyping is essential for lymphocytecloned classification, prognosis and treatment option in human and canine lymphomas. Immunohistochemistry (IHC) is a classic method that is referred to a gold standard due to its accuracy (Valli et al., 2011; Thalheim et al., 2013). Regarding tissue specimens, it provides morphology of lymph nodes (LN) for histological assessment, but generalized ane...

This article summarized immunohistochemistry methods generally used in research laboratories and clinic including direct immune staining, indirect immune staining, enzyme method, fluorescence method, APC method and PAP method.