The tests described hereafter will allow quantitative enumeration of mesophilic bacteria and fungi that may grow under aerobic conditions. The tests are designed primarily to determine whether a substance or preparation complies with an established specification for microbiological quality. When used for such purposes, follow the instructions given below, including the number of samples to be t...

It has been known for decades that microbial fermentation within the rumen is critical to postnatal rumen epithelial growth and maturation in ruminants, but the underlying mechanism is largely unknown. In this study, we determined the effect of rumen fluid, which should contain all products from rumen fermentation, on growth of rumen epithelial cells in vitro. Addition of 10% rumen fluid from c...

1. [3H]leucine infused directly into the rumen of sheep labelled microbial protein and, when compared with the specific activity of the rumen free-leucine pool, indicated that 50% of the bacterial protein leucine originated from the rumen free-leucine pool. 2. The lower limit for bacterial protein turnover in the rumen was 0.37/d when calculated as the difference between the specific rate of di...

There is an increasing need to identify alternative feeds for livestock that do not compete with foods for humans. Seaweed might provide such a resource, but there is limited information available on its value as an animal feed. Here we use a multi-omics approach to investigate the value of two brown seaweeds, Ascophyllum nodosum (ASC) and Laminaria digitata (LAM), as alternative feeds for rumi...

In this study, Lippia origanoides Kunth, native to the Alto Patía region in Southwest Colombia, and P ichia guilliermondii LV196, an inactivated yeast from germplasm bank of Agrosavia (Colombian Agricultural Research Corporation), alone or combined, were tested for their long-term effect on rumen fermentation methanogenesis whilst also characterising bacterial methanogen communities. Whereas es...

Assessment of the rumen fluid is an ancillary examination technique used in the clinical examination both of the individual bovine patient and at population level. The specific function and structure of the rumen makes ruminants different from other animals. Therefore, it logical to assess the rumen in detail when confronted with a bovine patient. Assessment of the rumen fluid is essential to a...

In our study the presence of bacteria, yeast, and microscopic fungi was evaluated. Three forms corn silage were made including without additive, with microbial additive (lactic acid bacteria), nutritional (urea). Silage additives applied to matter within recommended dosage, then ensiled into plastic bags stored at a constant temperature. After 5.5 months storage, average samples for mycotoxins ...

The study investigated microbial population dynamics in soils of Greater Port Harcourt Area, Nigeria. involved the quantification petroleum pollutants and enumeration characterization culturable fungi bacteria. Gas Chromatograph FID method was used to quantify total hydrocarbons (TPH) soil while morphological biochemical tests were characterize microorganisms. highest TPH 9,759.37±883.36...