1. Responses to twice-weekly oral supplementation with 4.0 g methionine were measured with lambs (27 kg) grazing kale (Brassica oleracea) for 10 weeks (Expt 1). In a second experiment with sheep fed on kale at hourly intervals, rumen fractional outflow rates of CrEDTA and ruthenium Tris(1,10 phenanthroline) markers were measured from the rates of decline in their concentrations. Rumen turnover ...

rumen is a persistent and specific ecosystem consists of bacteria, protozoa and fungus where feed fermentation takes place in it. produced hydrogen in rumen can be used in the synthesis of the volatile fatty acids and the microbial protein and its excess would be eliminated through the production of methane by methanogenesis. nutritionists have tried to find ways to decrease loss and energy and...

Degradation of plant N in the rumen results in the formation of ammonia-N and NAN. Rumen bacteria utilise these breakdown products to synthesise their cell proteins. This study was carried out to investigate the in vivo degradation of N-labelled duckweed in the rumen and the utilisation of the degradation products by rumen bacteria. We found that duckweed protein was relatively resistant to rum...

BACKGROUND Rumen microbes play a greater role in host energy acquisition than that of gut-associated microbes in monogastric animals. Although genome-enabled advancements are providing access to the vast diversity of uncultivated microbes, our understanding of variables shaping rumen microbial communities is in its infancy. Viruses have been shown to impact microbial populations through a myria...

Rumen nitrogen metabolism values were estimated by the use of a single injection of 15(NH4)2SO4 into the rumen of sheep and consecutive 15N enrichment measurements in the rumen ammonia pool, rumen non-NH3-N (NAN) pool, rumen purine pool and blood urea-N (BUN) pool for a period of 24 h. Synthesis and degradation of N compounds in the rumen and passage of N to and from the rumen were evaluated on...

The majority of workers are agreed that the digestion of carbohydrate in the rumen is brought about by the micro-organisms living there, and furthermore, that the microflora plays a much larger part in these processes than the microfauna, for if the Protozoa are removed by drenching with dilute copper sulphate, the digestive processes continued unhampered (see Van der Wath, 1942). The precise r...

High concentrations of the ciliate Epidinium Crawley are associated with damaged regions of fresh plant material undergoing digestion in the sheep rumen. This finding supports that postulate that sequestration in the rumen explains the low rate of passage of protozoa despite the high flow rate of liquid from the rumen. The maintenance of Epidinium in the rumen, despite their slow growth rate, i...

Local feed resources, particularly low-quality roughages and agricultural crop-residues, are of prime importance for ruminants raised in the tropics. These feeds exhibit close relationships with rumen ecology, microbes and rumen fermentation patterns. A number of dietary factors could influence rumen fermentation especially the basal roughage source, its physical form and fermentation end-produ...

Serotonin is an omnipotent molecule in the brain and rest of body plays a pivotal role gut-brain axis.Serotonin’s neurotransmission its function at molecular levels with different genetic variants has beenwidely covered across animal kingdom. Although digestion process ruminants pillared on anaerobicmicroflora present rumen, impact rumen microbial population serotonergic activity seldomexplored...

The aim of this work was to investigate the effect of feeding ethyl-3-nitrooxy propionate (E3NP) and 3-nitrooxypropanol (3 NP), 2 recently developed compounds with potential antimethanogenic activity, in vitro and in vivo in nonlactating sheep on ruminal methane production, fermentation pattern, the abundance of major microbial groups, and feed degradability. Three experiments were conducted, 1...