When reovirus-infected Hela cells are incubated at 43 degrees C virus-specific messenger RNA is released from the polysomes. It accumulates free in the cytoplasm as messenger ribonucleoprotein particles (mRNPs). These particles have a sedimentation rate of about 50S and a buoyant density in CsCL of 1.42 g/cm3. Reovirus mRNPs contain, besides all three size classes of reovirus messenger RNA, the...

A cDNA clone for the Ya subunit of glutathione transferase from rat liver was constructed in E. coli. The clone hybridized to Ya and Yc subunit messenger RNAs. On the basis of experiments involving cell-free translation and hybridization to the cloned probe, it was shown that prototype inducers of cytochrome P-450 such as phenobarbitone and 3-methylcholanthrene as well as inhibitors such as CoC...

The effect of prolonged benzodiazepine administration on GABAA receptor subunit (a1–6, b1–3, g2) messenger RNAs was investigated in the rat hippocampus and cortex, among other brain areas. Rats were orally administered flurazepam for one week, a protocol which results in benzodiazepine anticonvulsant tolerance in vivo, and in the hippocampus in vitro, in the absence of behavioral signs of withd...

Background: Atrial fibrillation (AF) is the most common complication in patients undergoing cardiac surgery. However, pathogenesis of postoperative AF (POAF) elusive, and research related to this topic sparse. Our study aimed identify key gene modules genes conduct a circular RNA (circRNA)-microRNA (miRNA)-messenger (mRNA) regulatory network analysis POAF on basis bioinformatic analysis.

background: rna interference (rnai) is the mechanism of gene silencing-mediated messenger rna degradation by small interference rna (sirna), which becomes a powerful tool for in vivo research, especially in the areas of cancer. in this research, the potential use of an expression vector as a specific sirna producing tool for silencing of bcr-abl in k562 cell line has been investigated. methods:...

There have been a number of attempts to direct protein synthesis using RNA particles containing specific genetic information. Although a variety of RNA preparations can stimulate polypeptide synthesis in bacterial cell-free systems, transfer of information has been demonstrable only with viral RNA or with synthetic polyribonucleotides.1-5 The ability to direct protein synthesis with either vira...

Chromatography on methylated albumin-kieselguhr of RNA from Saccharomyces cerevisiae was used to separate stable RNA from a tenaciously bound DNA-like RNA fraction. The tenaciously bound RNA, which was eluted with a dilute solution of sodium dodecyl sulphate, was characterized as messenger-like RNA by its sedimentation behaviour, nucleotide composition, lack of methylated bases and labelling ki...

BACKGROUND AND PURPOSE Cancer cells that express P-glycoprotein, multidrug resistance-associated protein (MRP), or lung resistance protein (LRP) have demonstrated resistance to a wide variety of chemotherapeutic drugs. Recently, we reported that human colon carcinoma cells that express all three proteins exhibit reduced P-glycoprotein gene expression and a loss of multidrug resistance after exp...

Gap junctions between vessel wall cells provide a pathway for the intercellular exchange of ions and small molecules. Pure cultures of microvascular and macrovascular endothelial and smooth muscle cells, vascular pericytes, and several nonvascular cell lines were tested for junctional communication by fluorescent dye transfer. All of the vascular wall cells were capable of dye transfer. Since g...