Although much is known about the mechanisms of signal-mediated protein and RNA nuclear import and export, little is understood concerning the nuclear import of plasmid DNA. Plasmids between 4.2 and 14.4 kilobases were specifically labeled using a fluorescein-conjugated peptide nucleic acid clamp. The resulting substrates were capable of gene expression and nuclear localization in microinjected ...

Infection of non-dividing cells is a biological property of HIV-1 crucial for virus transmission and AIDS pathogenesis. This property depends on nuclear import of the intracellular reverse transcription and pre-integration complexes (RTCs/PICs). To identify cellular factors involved in nuclear import of HIV-1 RTCs, cytosolic extracts were fractionated by chromatography and import activity exami...

Xylose-to-xylitol bioconversion by Ca-alginate entrapped Candida guilliermondii cells in sugarcane bagasse hemicellulosic hydrolysate was carried out in erlenmeyer flasks using the repeated-batch mode of fermentation. The hydrolysate was supplemented or not with ammonium sulfate and/or rice bran extract at the beginning of each repeated batch. Altogether, six sets of three repeated-batches were...

in recent decades, theorists proposed the role of domestic components such as interior active groups, policies and macroeconomic indicators on determination of protection policies. in the context of recent studies, this study has investigated the effect of business cycle fluctuations on import protection for selected developing countries in 1995-2011 by using dynamic panel data method. furtherm...

In the present innovative pursuit, the generalized Nörlund summability method, that is, the (N, c, d) summation process of Fourier series of f (x) as well defined by Borwein [4] has been invoked to approximate the function f (x) belonging to Lip M α (0 < α < 1 ) and M being some constant. Here it is worth-emphasising that not only mere approximation, but also the constants appeared in the proce...

In this paper, some new properties of the fundamental solution to the multi-dimensional 1 spaceand time-fractional diffusion-wave equation are deduced. We start with the Mellin-Barnes 2 representation of the fundamental solution that was derived in the previous publications of the 3 author. The Mellin-Barnes integral is used to get two new representations of the fundamental solution 4 in form o...

Nuclear import of some viral proteins depends on importin a proteins. However, no preferences of distinct a-importins for any viral protein import have been demonstrated. We used in vitro import assays and observed that all ubiquitously expressed human importin a isoforms mediate nuclear translocation of adenoviral E1A. Competition with nucleoplasmin suggests that importin a3 is the most effici...