A field experiment was conducted during Rabi season 2021-22 at the experimental of Crop Research Farm, Department Agronomy, Naini Agricultural Institute, Sam Higginbottom University Agriculture, Technology And Sciences, Prayagraj, Uttar Pradesh. India which is located 250 30’ 42’’N latitude, 810 60’ 56” E longitude, and a height 98 meters above sea level. The Experiment laid out in Randomized B...

Dit onderzoek is uitgevoerd binnen de onderzoekschool Experimentele Planten Wetenschappen (EPS) Nod factor signaling and infection in Rhizobium-legume symbiosis

Many animal-pathogenic bacteria can use heme compounds as iron sources. Like these microorganisms, rhizobium strains interact with host organisms where heme compounds are available. Results presented in this paper indicate that the use of hemoglobin as an iron source is not restricted to animal-pathogenic microorganisms. We also demonstrate that heme, hemoglobin, and leghemoglobin can act as ir...

Rhizobium strains nodulating green gram [Vigna radiata (L.) Wilczek] were found to produce bacteriocin on modified Bergersen's medium and inhibited the growth of homologous Rhizobium strains. Four bacteriocin producing and four bacteriocin non-producing strains were compared for their effect on nodulation, in planta nitrogenase activity and plant dry weight of green gram. The bacteriocin produc...

Plant growth promoting rhizobacteria (PGPR) are known to influence plant growth by various direct or indirect mechanisms. In search of efficient PGPR strains with multiple activities, a total of 150 bacterial isolates belonging to Bacillus, Pseudomonas, Azotobacter and Rhizobium were isolated from different rhizospheric soil of chick pea in the vicinity of Allahabad. These test isolates were bi...

Dot immunablot assay was used for determination of nodules produced on soybean. french bean. pigeon pea and urdbean by Bradyrhizobiumjaponicum USDA-I 10; R. leguminosarum bv. phaseoli FB-77 and N-3; Rhizobium sp. A-3 and U-I. respectively. Nodule occupancy by inoculated strains as determined by the test ranged between 73 to 93%. Replica inmrunoblot assay reduced the time required for enumeratio...

Rhizobium leguminosarum bv. viciae Exo- mutant strains RBL5523,exo7::Tn5,RBL5523,exo8::Tn5 and RBL5523,exo52::Tn5 are affected in nodulation and in the syntheses of lipopolysaccharide, capsular polysaccharide, and exocellular polysaccharide. These mutants were complemented for nodulation and for the syntheses of these polysaccharides by plasmid pMP2603. The gene in which these mutants are defec...

Rhizobium spp. show chemotaxis to plant root exudates. Both legumes and non-legume root exudates attract the different rhizobia studied. However, the bacteria show a differential response in that they are attracted to the root exudates of some plants and show no attraction toward others. An example of negative chemotaxis was also observed. The trefoil strain of Rhizobium shows chemotaxis which ...