BACKGROUND Tinea cruris is the second most common dermatophytosis in the world and the most common in Indonesia. The conventional laboratory tests for dermatophyte infection are slow and less specific. Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) is a PCR method with the addition of enzyme after amplification, therefore enabling for more specific results. AIM ...

A combined RFLP and AFLP map was constructed for hexaploid oat (Avena spp.). The segregation of AFLP markers was scored in two hexaploid oat recombinant inbred line (RIL) populations, the 'Kanota' x 'Ogle' RFLP population, and a population derived from 'Clintland64' and 'IL86-5698', barley yellow dwarf virus (BYDV)-sensitive and BYDV-tolerant lines, respectively. More than 300 AFLP markers were...

Single nucleotide polymorphisms (SNPs) can be genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Before performing PCR-RFLP for SNP genotyping, a feasible primer pair observes numerous constraints and an available restriction enzyme discriminate the target SNP, are required, here we called it natural SNP-RFLP primer design. Design the natural SNP-RFLP pr...

DNA genotyping of Mycobacterium tuberculosis has been widely applied in the understanding of disease transmission in many countries. The purpose of this study was to genotype the strains of M. tuberculosis isolated in patients with new tuberculosis (TB) cases in Minas Gerais, as well as to compare the similarity, discriminatory power, and agreement of the clusters between the IS6110 Restriction...

BACKGROUND The intergeneric hybrids between Ascocenda John De Biase 'Blue' and Phalaenopsis Chih Shang's Stripes have been generated to introduce the blue color into the Phalaenopsis germplasm in prior study. In order to confirm the inheritance in hybrid progenies, genomic in situ hybridization (GISH) and restriction fragment length polymorphism (RFLP) analysis were conducted to confirm the int...

Salinity is one of the most critical problems for agricultural development and threatens future food safety. Therefore, we aimed to investigate root application zinc oxide nanoparticles (ZnO-NPs; 0, 50, 100 mg/L), 24-epibrassinolide (EBL; 0.02, 0.04 µM), their combinations on growth performance maize (Zea mays L.) as a model plant grown under salt stress (i.e., 5 10 dS m−1) in hydroponic system...

Objective: The objective of this study was to investigate the group/subgroup phytoplasma agent in peppers showing symptoms. Material and Methods: In study, plants collected from Iğdır province 2020 were analyzed using direct nested PCR tests, BLASTn, iphyClassifier, Mega 7, pDRAW32 programs used. Results: tests performed, approximately 1.2 kb DNA fragments specific obtained. 16S rRNA nucleotide...

BACKGROUND AND OBJECTIVES In Iran, anaplasmosis is normally diagnosed with traditional Giemsa staining method. This is not applicable for identification of the carrier animals. The aim of this study was to compare the detection of Anaplasma marginale in two different numbers of microscopic fields (50 and 100) using conventional Giemsa staining method compared with the PCR-RFLP technique. MATE...

Bambara groundnut (Vigna subterranea (L.) Verdc) is a nutritious and drought-tolerant crop that native to Africa. Currently, it possesses cosmopolitan distribution across the semi-arid agro-ecologies of Africa its cultivation has extended other regions, particularly in drier parts Asia Indonesia. Due historic neglect, research on policy prioritization national breeding programs lagging; hence, ...