Article type: Original article Objective(s): The utilization of molecular techniques in the epidemiology of tuberculosis have provided an opportunity for using effective markers to trace the transmission of the disease .The purpose of this study was to compare the genetic patterns of Mycobacterium tuberculosis by three methods of RFLP technique. Materials and Methods: In a cross-sectional and p...

OBJECTIVES The utilization of molecular techniques in the epidemiology of tuberculosis have provided an opportunity for using effective markers to trace the transmission of the disease. The purpose of this study was to compare the genetic patterns of Mycobacterium tuberculosis by three methods of RFLP technique. MATERIALS AND METHODS In a cross-sectional and prospective study, 95 strains of M...

In the presented paper, PCR-RFLP and RFLP-PFGE with two different enzymes were applied for the discrimination of 48 Campylobacter isolates from poultry carcasses and faeces. Both used typing methods had a typeability of 100% on this collection of the strains. PFGE with SacII enzyme was the most discriminatory method with the discrimination index of 0.98. Restriction analysis of the flaA gene al...

Assessing microbiological quality assurance by monitoring bacteria in various sources of freshwater used for human consumption, recreation, and food preparation is important for a healthy life. Bacterial number and their community structure in freshwater should be determined as quickly as possible, and "real-time" and "on-site" microbiological methods are required. In this study, we examined th...

BACKGROUND Onychomycosis is a fungal infection of one or more units of the nail caused by dermatophytes, or mould and nondermatophytes yeast. Investigations are needed to establish the diagnosis of onychomycosis before starting treatment. Several investigations methods for diagnosing onychomycosis are microscopic examination with 20% KOH, fungal culture, histopathology examination with PAS stai...

A maximum likelihood approach of half tetrad analysis (HTA) based on multiple restriction fragment length polymorphism (RFLP) markers was developed. This procedure estimates the relative frequencies of 2n gametes produced by mechanisms genetically equivalent to first division restitution (FDR) or second division restitution and simultaneously locates the centromere within a linkage group of RFL...

BACKGROUND It is clinically important to detect and type human papillomavirus (HPV) in a sensitive and specific manner. OBJECTIVES Development of a nested-polymerase chain reaction-restriction fragment length polymorphism (nested-PCR-RFLP) assay to detect and type HPV based on the analysis of L1 gene. METHODS Analysis of published DNA sequence of mucosal HPV types to select sequences of new...