Blastomycosis is a serious and potentially fatal infection caused by the thermally dimorphic fungus Blastomyces dermatitidis. Polymerase chain reaction (PCR) assays targeting the BAD-1 virulence gene promoter have been developed to aid in the detection of the pathogen in clinical and environmental samples. However, little is known regarding the genetic diversity of B. dermatitidis and how this ...

detection of mycoplasma synoviae (ms) by culture and polymerase chain reaction (pcr) has been reported from commercial chicken farms in different provinces of iran. in some reports the phylogenetic analysis of ms isolates based on 16s rrna and variable lipoprotein hemagglutinin (vlha) genes have been carried out. the pcr product containing partial 16s rrna genes of iranain isolates was sequence...

introduction: different isolates of human specifies, giardia intestinalis, also known as giardia lambia, have been shown to vary in sensitivity to drug, isoenzyme pattern, virulence and infectivity, antigens and restriction enzyme pattern. material and methods: using system that distinguishes sequence differences in the metabolic enzyme tpi, dna extracted from trophozoite that culture in modifi...

Several yeasts were isolated from Campbell Early grapes (Vitis labrusca cultivar Campbell Early), the major grape cultivar in Korea, grown in two different regions. PCR-RFLP analysis of the ITS I-5.8S-ITS II region showed that 34 isolates out of a total of 40 were in the same group. Phylogenetic analysis revealed that the major strain belonged to one species, Hanseniaspora uvarum, although they...

Phytoplasma-like symptoms of leaf yellowing and calyx malformation were observed in eggplant (Solanum melongena L.), upward leaves fruit pepper (Capsicum annuum aerial tuber formation potato (S. tuberosum L.) during the survey performed late season (August to September) 2015 2016 Van province (Turkey). A total 100 samples tested by nested-PCR using universal primer pairs assess sanitary status ...

Two hundred twenty-nine consecutive isolates of Mycobacterium tuberculosis complex from patients with pulmonary tuberculosis in Guinea-Bissau, which is located in West Africa, were analyzed for clonal origin by biochemical typing and DNA fingerprinting. By using four biochemical tests (resistance to thiophene-2-carboxylic acid hydrazide, niacin production, nitrate reductase test, and pyrazinami...

A total of 73 isolates (57 Enterobacter cloacae and 16 Enterobacter agglomerans), recovered during an outbreak of bacteremia in the Campinas area, São Paulo, Brazil, were studied. Of these isolates, 61 were from parenteral nutrition solutions, 9 from blood cultures, 2 from a sealed bottle of parenteral nutrition solution, and one was of unknown origin. Of the 57 E. cloacae isolates, 54 were bio...

Molecular markers provide an important method for detecting tuberculosis (TB) transmission that has been followed by rapid progression to active disease. Restriction fragment length polymorphism (RFLP) typing of Mycobacterium tuberculosis has been used to confirm outbreaks, through the demonstration of shared isolates. In population-based studies, it has therefore been inferred that patients wi...

Restriction fragment length polymorphism (RFLP) analysis of isolates of Cryptosporidium parvum has revealed two subgroups, termed H and C. The limited resolution of the RFLP method precludes an in-depth study of the genetic structure of C. parvum populations. Published C. parvum restriction polymorphisms lie within protein-coding regions known to be more homogeneous than noncoding sequences. To...