The reversible inhibitory effects of nitric oxide (.NO) on mitochondrial cytochrome oxidase and O(2) uptake are dependent on intramitochondrial.NO utilization. This study was aimed at establishing the mitochondrial pathways for.NO utilization that regulate O-(2) generation via reductive and oxidative reactions involving ubiquinol oxidation and peroxynitrite (ONOO(-)) formation. For this purpose...

The newly found B40 is the first experimentally observed all-boron fullerene and has potential applications in hydrogen storage. Here we investigate the binding ability and hydrogen storage capacity of Ti-decorated B40 fullerene based on DFT calculations. Our results indicate that Ti shows excellent binding capability to B40 compared with other transition metals. The B40 fullerene coated by 6 T...

Porphyromonas gingivalis 381 cells were incubated with 125I-histidine-rich polypeptide (histatin) 5 in the presence or absence of unlabeled histatin 5, to evaluate the histatin-binding capacity of the cells. The binding of histatin 5 was rapid, reversible, saturable and specific. The number of histatin 5-binding sites per cell was 3,600, and the dissociation constant (Kd) was in the order of 10...

Putative receptors for arginine-vasopressin (AVP) in rat brain were localized and characterized using autoradiographic and radioligand-binding techniques. When brain slices were incubated with tritium-labeled vasopressin, an intense concentration of binding sites was identified which included the medial aspects of the amygdala. Binding of labeled AVP to membrane preparations from this brain reg...

To understand how multiprotein complexes assemble and function on chromatin, we combined quantitative analysis of the mammalian nucleotide excision DNA repair (NER) machinery in living cells with computational modeling. We found that individual NER components exchange within tens of seconds between the bound state in repair complexes and the diffusive state in the nucleoplasm, whereas their net...

The electronic structure of the heme iron in hemoproteins is characterized by its changeable valence and spin states. These features are discussed in hemoproteins and their related model compounds. The iron-ligand binding, especially the reversible dioxygen binding in myoglobin and hemoglobin is discussed in details by using the f4;issbauer spectroscopic knowledge obtained from the experiments ...

Previously published research used an isotope-exchange technique to measure irreversibility of pesticide sorption-desorption in soil. Results indicated significant irreversibility (6-51%) in sorption in five pesticide-soil systems measured over 72 h. Here, we propose a three-site model to reanalyze the experimental data. The model adds a slow but reversible binding on nonequilibrium sorption si...

Dynamic chemical devices involve morphological or constitutional modifications in molecular or supramolecular systems, induced by internal or external physical or chemical triggers. Reversible changes in shape result in molecular motions and define motional dynamic devices presenting mechanical-like actions of various types. Suitably designed polyheterocyclic strands such as compounds 1-5 wrap ...

Glyceraldehyde-3-phosphate dehydrogenase was found to bind in vitro to purified, human erythrocyte glucose transporter reconstituted into vesicles. Mild tryptic digestion of the glucose transporter totally inactivated the binding, suggesting that the cytoplasmic domain of the transporter is involved in the binding to glyceraldehyde-3-phosphate dehydrogenase. The binding was abolished in the pre...

Graphical techniques provide simple methods for the analysis of data from tracer studies. They provide considerable ease of computation compared to the optimization of individual model parameters in the solution of the differential equations generally used to describe the binding of tracers. The theoretical work of Patlak which was applied to irreversible tracers formed the basis for extensions...