نتایج جستجو برای: reverse transcriptase polymerase chain reaction

تعداد نتایج: 757054  

2015
Waikhom Bimolata Anirudh Kumar Sai Kiran Reddy M Raman Meenakshi Sundaram Gouri Sankar Laha Insaf Ahmed Qureshi Irfan Ahmad Ghazi

Nucleotide sequence polymorphisms among R gene alleles influence the process of co-evolutionary interaction between host and pathogen by shaping the response of host plants towards invading pathogens. Here, we present the DNA sequence polymorphisms and diversities present among natural alleles of three rice bacterial blight resistance genes, Xa21, Xa26 and xa5. The diversity was examined across...

Journal: :Applied and environmental microbiology 2009
Scott R Clingenpeel Seth D'Imperio Harry Oduro Greg K Druschel Timothy R McDermott

Novel arsenite [As(III)] oxidase structural genes (aoxAB) were cloned from Hydrogenobaculum bacteria isolated from an acidic geothermal spring. Reverse transcriptase PCR demonstrated expression throughout the outflow channel, and the aoxB cDNA clones exhibited distribution patterns relative to the physicochemical gradients in the spring. Microelectrode analyses provided evidence of quantitative...

Bovine viral diarrhea virus (BVDV) is a pathogen that infects cattle, and is globally important. It causes substantial financial losses to the livestock industry. In the current study, a one-step reverse transcriptase-loop-mediated isothermal amplification (RT-LAMP) assay was set up for rapid and efficient detection of BVDV. For this purpose, four primers were designed to recognize six distinct...

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