نتایج جستجو برای: reverse phase liquid chromatography
تعداد نتایج: 923386 فیلتر نتایج به سال:
Rapid and sensitive reverse phase high performance liquid chromatography (RP-HPLC) and ultra performance liquid chromatography (UPLC) methods with UV detection for quantification of erythropoietin (EPO) in presence of human serum albumin (HSA) as a stabilizer in a pharmaceutical formulation of EPO have been developed and validated. Chromatography was performed with mobile phase containing 0.1% ...
We describe the use of electron-capture gas chromatography or reversed-phase "high-performance" liquid chromatography for concurrent analysis for diazepam and nordiazepam in serum. In the gas-chromatographic analysis our use of a new chemically deactivated stationary liquid phase, SP 2250-DB (Supelco, Inc.), resulted in improved chromatographic sensitivity and peak symmetry for the two benzodia...
This paper describes a high-performance liquid chromatography method for the determination of atenolol in human plasma. Atenolol and the internal standard, metoprolol, were extracted from plasma by using a liquid-liquid extraction method. The method was developed on an Ace C18 reverse-phase column using a mobile phase of methanol-water (50:50, v/v) containing 0.1% trifluoroacetic acid. The cali...
The current wound-healing collagen mimetic peptides (CMPs) have limitations such as poor membrane permeability and protease susceptibility. Herein, the solid-phase peptide synthesis of CMPs containing integrin binding motif GFOGER is reported. sequences also consist lipophilic moieties (adamantane palmitic acid) for improved different collagen-inducing tripeptides, namely, Thr-Thr-Lys (TTK), Gl...
There is no other naturally occurring defense agent against cancer that has a stronger effectthan paclitaxel, commonly known under the brand name of Taxol®. The major drawback for themore widespread use of paclitaxel and its precious precursor, 10-deacetylbaccatin III (10-DABIII), is that they require large-scale extraction from different parts of yew trees (Taxus species),cell cultures, taxane...
Dipicolinic acid was determined by reverse-phase liquid chromatography. Elution was with 0.2 M potassium phosphate, pH 1.8, containing 1.5% tert-amyl alcohol or higher concentrations of lower alcohols or acetonitrile. The normal analytical range was 50 to 1,000 muM, which is equivalent to 0.1 to 1 mg of spores per ml with a relative standard error of 2 to 4% and a detection limit of <100 pmol. ...
To better understand the molecular mechanisms underpinning physiological variation in human populations, metabolic phenotyping approaches are increasingly being applied to studies involving hundreds and thousands of biofluid samples. Hyphenated ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) has become a fundamental tool for this purpose. However, the seemingly inevitable ne...
results under the above chromatography condition, biuret compound (peqb) and the internal standard were detected at 4.5 and 13.5 minutes, respectively. limit of quantitation of the peqb was 0.1 µg/ml. accuracy of the method over the concentration range of 0.1-100 µg/ml was between 88-109%. inter- and intraday precisions were 4-19% and 6-8%, respectively. a good relationship in the form of a pow...
An impurity of Ampicillin sodium formed during a gradient reverse phase high performance liquid chromatography (HPLC) analysis of stress stability samples of the drug substance Ampicillin sodium, and the level of this impurity was found at up to 0.9%. This impurity was identified by LC-MS and characterized by (1H NMR, LC/MS/MS, elemental analysis). Based on the spectral data, the impurity was n...
We describe an assay system for measuring phenobarbital, diphenylhydantoin, primidone, ethosuximide, and carbamazepine in 25 mul of serum. The procedure involves precipitation of proteins with an acetonitrile solution containing cyheptamide as an internal standard, and reverse-phase chromatography on a 4 mm X 30 cm column containing "muBondapak C18." The anticonvulsants are eluted with an equiv...
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