The detection of bacterial DNA in serum and ascitic fluid (AF) from patients with liver cirrhosis and ascites is interpreted as molecular evidence of intestinal bacterial translocation (BT) and considered sufficient to activate the cellular immune response leading to greater cytokine synthesis. We studied 34 patients with liver cirrhosis and culture-negative, non-neutrocytic ascites [22 patient...

Objective: Terminalia arjuna is commonly known as Arjuna and widely used as cardioprotective agent in Indian traditional medicine. The present study was undertaken to evaluate the protective effect of ethanolic extract of T. arjuna bark (TAA) and its fractions, including dichloromethane (TAD), ethyl acetate (TAE), butanol (TAB) and water (TAW) against free radicals, protein oxidation and DNA da...

Nuclear preparations from the Walker tumor were isolated after homogenization of the tumors in 2.5% citric acid. The re covery of nuclei in the procedure employed was 43%, and the purity of the preparations was 85-90% by particle counts with the aid of phase microscopy. The DNA, RNA, and protein con tents of the isolated tumor nuclei were 11.9, 5.7, and 34.4 pg per nucleus, respectively. The RN...

In order to determine whether the covalent binding of the carcinogen 1,2-dichloroethane to macromolecules is dependent on microsomes or cytosol, microsomes and cytosol from lungs of C57BL/6 X C3H/He F1 (hereafter called B6C3F1) mice and Osborne-Mendel rats were incubated with [1,2-14C]dichloroethane and salmon sperm DNA. 1,2-Dichlorothane binds covalently to microsomal protein and DNA only in t...

Cytomegalovirus (CMV) DNA quantitation in clinical specimens is progressively becoming a cornerstone in the diagnosis and management of CMV infection in the immunocompromised host. We evaluated two automated and reproducible PCR tests, the LightCycler (Roche Molecular Biochemicals, Indianapolis, Ind.) and the COBAS AMPLICOR CMV Monitor (Roche Diagnostics, Pleasanton, Calif.), for the detection ...

The primary objective of the present study was to test whether agricultural chemical runoff was associated with in-stream genotoxicity in native fish. Using Sacramento sucker (Catostomus occidentalis), we combined field-caging experiments in an agriculturally dominated watershed with controlled laboratory exposures to field-collected water samples, and we coupled genotoxicity biomarker measurem...