BACKGROUND In this study, two-dimensional gel electrophoresis (2-DE) method was applied to determine and compare the protein spots expressed in the two field isolates of Leishmania major and recovered from the patients who were clinically sensitive and resistant to Glucantime® treatment. METHODS Leishmania parasites were isolated from the cutaneous lesions of two CL infected patients in Shira...

in order to evaluate of morphological, physiological and biochemical traits, related to drought tolerance, an experiment was carried out in field condition. in this study two commercial cultivars (mcc361, mcc358), three kabuli and three deci type of tolerant genotypes (mcc392, mcc537, mcc696, mcc873, mcc870, mcc10,) and three kabuli and three deci type of susceptible genotypes (mcc759, mcc588, ...

Abstract Bread wheat ( Triticum aestivum L.) is the most widely grown crop in world. Rhizoctonia cerealis , causal agent of sharp eyespot disease, has 21 become epidemic many countries. In present study, we performed transcriptome analysis infected by R. at 0, 12, 30, 70, and 98 h post-infection using -resistant -susceptible genotypes (CI12633 ‘Yangmai15’, respectively). We used quantitative re...

Glutamate, as an essential neurotransmitter, has been thought to have different roles in the central nervous system (CNS), including nerve regeneration, synaptogenesis, and neurogenesis. Excessive glutamate causes an up-regulation of the multiple signaling pathways, including phosphoinositide-3 kinase/protein kinase B (PI3K/Akt), Akt/mammalian target of rapamycin (mTOR) protein, mitogen-activat...

The objectives of this study were to assess the genotype (G) by environment (E) interaction of grain yield of durum wheat(Triticum durum Desf.) based on parametric models, additive main effects and multiplicative interaction (AMMI) and joint linear regression models; and compare the relative efficiency of the two models in explaining the GE effects. Twenty-three genotypes were evaluated across ...

Sphingomonas sp. strain RB2256 is representative of the ultramicrobacteria that proliferate in oligotrophic marine waters. While this class of bacteria is well adapted for growth with low concentrations of nutrients, their ability to respond to complete nutrient deprivation has not previously been investigated. In this study, we examined two-dimensional protein profiles for logarithmic and stat...

although self incompatibility in walnut has not been reported, but the dicogamy limits self pollination of walnut cultivars. as the density of female flowers produced by the tree is low, if a high fruit set occurs, yield especially in terminal bearing cultivars will not be high. so breeding of a pollinizer cultivar with proper pollen shading period is highly necessary for increasing fruit produ...

membrane/ protein trafficking in the secretory/ biosynthetic and endocytic pathways is mediated by vesicles. vesicle trafficking in eukaryotes is regulated by a class of small monomeric gtpases the rab protein family. rab proteins represent the largest branch of the ras superfamily gtpases, and have been concerned in a variety of intracellular vesicle trafficking and different intracellular sig...

MicroRNAs are key regulators associated with numerous diseases. In HEK293 cells, miR-153-3p and miR-205-5p down-regulate alpha-synuclein (SNCA) and Leucine-rich repeat kinase 2 (LRRK2), two key proteins involved in Parkinson's disease (PD). We have used two-dimensional gel electrophoresis (2D-PAGE) coupled to mass spectrometry (MS) to identify a spectrum of miR-153-3p and miR-205-5p targets in ...