Peppers (Capsicum spp.) are recalcitrant to in vitro culture regeneration, making the application of vitro-based breeding strategies difficult. We evaluated impact different combinations auxins, cytokinins and micronutrients on induction direct organogenesis cotyledon hypocotyl explants C. annuum, baccatum chinense. found variation regeneration response among species type explant. In this way, ...

genetic transformation studies were carried out to standardize a protocol for agrobacterium -mediated genetic transformation of three economically important strawberry (fragaria x ananassa duch) cultivars kurdistan’, camarosa’, and ‘paros’. shoot regeneration frequency 72, 65 and 30% was obtained on ms (1) basal medium supplemented with 2% glucose and 4 mg/l tdz for camarosa, kurdistan and paro...

A preparation of two commercial enzymes was used to liberate protoplasts from 16-h-old mycelium of Phanerochaete chrysosporium. Regeneration frequencies of up to 5% were attained when the protoplasts were plated in a medium containing 10% sorbose and 3% agar. Fusion of protoplasts from different auxotrophic strains in polyethylene glycol-Ca produced heterokaryons. Separation of the heterokaryon...

Muscari armeniacum is one of the important ornamental cut flower in floriculture industry which native to Southern Europe, North Africa, Western Asia and Asia Minor. In this study, bulb explants (basal plate of bulb having meristem), bulb scales and leaf segments from in vitro derived bulblets were culture in Murashige and Skoog (MS) medium with different plant growth regulato...

A protocol was developed for high frequency in vitro multiplication of an ornamental orchid, Phalaenopsis amabilis (L.) Blume var. Jawa, using plant growth regulators (PGRs). Protocorm-like bodies (PLBs), as explants were cultured on Murashige and Skoog (MS) medium fortified with various concentrations of kinetin (KIN) and indole-3-butyric acid (IBA), either individually or in...

In vitro propagation of C. morifolium cv. ‘Homa’ and cv. ‘Delkash’ and wild C. coccineum via direct and indirect organogenesis and somatic embryogenesis were investigated. BAP at 0, 1, 2 and 3 mg l-1 or NAA at 0, 0.05, 0.1 and 0.2 mg l-1 concentrations were used to induce direct and indirect organogenesis of shoot tip explants. To study the callu...

So far there is no information about receiving red beet androgenic embryos by androgenesis. Several factors were tested which affected this process: starch accumulation in microspores, correlation between bud length and microsporogenesis course, induction and regeneration medium composition. Ploidy level of obtained regenerants were evaluated. Treating anthers with α-amylase or watering donor p...