Latex exploitation-caused latex flow is effective in enhancing latex regeneration in laticifer cells of rubber tree. It should be suitable for screening appropriate reference gene for analysis of the expression of latex regeneration-related genes by quantitative real-time PCR (qRT-PCR). In the present study, the expression stability of 23 candidate reference genes was evaluated on the basis of ...

Obtaining quantitative data concerning gene expression is important for understanding milk synthesis in mammary glands. Quantitative real-time PCR (qRT-PCR) is an efficient tool to calculate gene expression; however, it is necessary to find valid reference genes for normalization of qRT-PCR data. We applied the geNorm software to eight commonly used reference genes to identify the most stable a...

Bone marrow mesenchymal stem cells (BMSCs) are considered as multipotent cells, representing a multi-lineage potential to differentiate into mesodermal lineages of mesenchymal tissues, including cartilage, bone, fat, muscle and tendon. Tissue engineering in BMSCs has made great advances in the regeneration of cartilage and bone defects. To uncover the mechanisms of the multipotent differentiati...

pedigree information of iranian holstein dairy cattle, collected via animal breeding centre of iran from 1971 to 2007, were utilized to describe the reasons for the increasing inbreed coefficient. inbreeding coefficient (f) and pedigree completeness (pci) of all the available cattle were found out using a pedigree structure of 883713 animals. the herds with pci of above 0.85 were picked out and...

background and aims: rubella virus is a human pathogen that causes congenital rubella syndrome (crs) when infection occurs during early pregnancy. vaccination programs have been remarkably successful in controlling natural rubella infection and crs. moreover, ongoing surveillance for all cases of rubella and crs is a vital component of a prevention program. although the who recommends the use o...

Cell differentiation depends mainly on specific mRNA expression. To quantify the expression of a particular gene, the normalisation with respect to the expression of a reference gene is carried out. This is based on the assumption that the expression of the reference gene is constant during development, in different cells or tissues or after treatment. Xenopus laevis studies have frequently use...