objective(s): despite treatment with antibiotics and vaccination with bcg, tuberculosis (tb) is still considered as one of the most important public health problems in the world. therefore, designing and producing a more effective vaccine against tb seems urgently. in this study, immunogenicity of a fusion protein which consisting or comprising cfp-10 from mycobacterium tuberculosis and the fc-...

The recombinant antibody H90-10 detects the endogenous human heat-shock protein 90 beta (Hsp90β) by immunoprecipitation (IP) and Western blotting.

background: infections due to pseudomonas aeruginosa are among the leading causes of morbidity and mortality in patients who suffer from impaired immune responses and chronic diseases such as cystic fibrosis. at present, aggressive antibiotic therapy is the only choice for management of p. aeruginosa infections, but emergence of highly resistant strains necessitated the development of novel alt...

Objective(s): Hydatidosis is a zoonotic infection and endemic in Iran. Due to the serological cross-reactivity (of sera) with other parasitic infection, diagnosis of hydatid cyst is considered to be problematic. In this regard, application of recombinant antigens improves serological diagnosis for human hydatidosis. Here, we present an ELISA test based on B8/2 recombinant antigen of Echinococcu...

expression of htlv-i p19 protein in an escherichia coli expression system always leads to the formation of inclusion body. solubilisation and refolding of the inclusion bodies is complex, time consuming and difficult during large-scale preparation. this study aimed to express and purify a soluble form of recombinant htlv-i p19 protein in an e. coli expression system. the synthetic dna encoding ...

A sweet water-soluble protein that reminds stable over wide ranges of temperature and pH, Brazzein has various applications. Its tastes like cane sugar but have no calories. However, the extraction of brazzein from its natural source is expensive and not applicable. In this study we used recombinant DNA technology to provide an alternative option for cheaper mass production of brazzein. A brazz...

The recombinant antibodies AS739, AT693, AU197 and AU734 detect by western blot the spike S protein from SARS-CoV-2.

The recombinant antibodies RB740, RB741 and RB742 detect by ELISA the Dictyostelium discoideum Talin-B fused to a GST protein.

The recombinant antibodies RB706, RB707, RB708, RB709, RB710 and RB711 detect by ELISA a synthetic peptide from the ROR2 protein.

background: enterotoxigenic escherichia coli (etec) strains are the major causes of diarrheal disease in humans and animals. colonization factors and enterotoxins are the major virulence factors in etec pathogenesis. for the broad-spectrum protection against etec, one could focus on colonization factors and non-toxic heat labile as a vaccine candidate. methods: a fusion protein is composed of a...