For the diagnosis of allergic aspergillosis demonstration of specific immune responses to allergens has been accepted as a significant paradigm. Elevated levels of total IgE and Aspergillus fumigatus specific IgE and IgG antibodies are important criteria for diagnosis of ABPA. Although reference antigens or standardized methods are not available, there are a number of relevant recombinant antig...

Background Mycoplasma hyopneumoniae is the primary etiologic agent of Swine Enzootic Pneumonia (EP), one of the most common respiratory disease that affects swine worldwide, causing considerable economic losses. Vaccination constitute one of the main practices to control EP. The production of recombinant experimental vaccines against EP has been considered an important approach towards the deve...

Toxoplasma gondii is a parasitic protozoan which is the cause of toxoplasmosis. Although human toxoplasmosis in healthy adults is usually asymptomatic, serious disease can occur in the case of congenital infections and immunocompromised individuals. Furthermore, despite the exact recognition of its etiology, it still presents a diagnostic problem. Diagnosis of toxoplasmosis is mainly based on t...

Background: Transient and stable transformation of host plants are the common techniques to produce transgenic plants. However, the main drawback of stable transformation is the fact that it takes quite a long time to produce a transgenic line. While, transient gene expression is a quick method to produce recombinant proteins in plants.  Objective: The main goal of the present study was to eva...

Trypanosoma cruzi antigens TSA-1 and Tc24 have shown promise as vaccine candidates in animal studies. We evaluated here the recall immune response these antigens induce in Chagasic patients, as a first step to test their immunogenicity in humans. We evaluated the in vitro cellular immune response after stimulation with recombinant TSA-1 (rTSA-1) or recombinant Tc24 (rTc24) in mononuclear cells ...

detection of plasmodium vivax specific antibodies with serological tests could be a valuable tool for epidemiological researches. whereas p. vivax cannot be simply obtained in vitro, serological tests using total or semi-purified antigens are infrequently used. given this restriction, the present study investigated whether recombinant p. vivax merozoite surface protein 1 (pvmsp-1 42 kda) could ...

brucellosis, caused by brucella spp., is an important zoonotic disease that causes abortion and infertility in cattle and undulant fever in humans. various studies have examined cell-free native and recombinant proteins as candidate protective antigens in animal models. among brucella immunogenes, antigen based on ribosomal preparation has been widely investigated. in this study, the immunogeni...

Facets of virulence for ferrets of 16 recombinant clones of two parent viruses A/Finland/4/74 (H3N2) and A/Okuda/57 (H2N2) were determined and viewed in relation to their genetic composition. Of the five pairs of recombinant clones with RNA segments of the same parental origin, differences in facets of virulence were detected between members of two of the pairs. One pair differed in ability to ...

Background: Dense granule antigens (GRA antigens) of Toxoplasma gondii induce strong antibody response in humans and are considered as useful diagnostic antigens. Previous studies reported expression of amino terminal GRA8 protein in fusion with large tags such as glutathione-S-transferase. The present study aimed to produce soluble full length immunogenic GRA8 in bacteria. Methods: GRA8 comple...

Full-length cDNA clones of the nucleoprotein (NP) genes of influenza A/Ann Arbor/6/60 and B/Ann Arbor/1/86 viruses were constructed from virion RNA and subsequently expressed in Spodoptera frugiperda (Sf9) cells using the baculovirus vector, Autographa californica nuclear polyhedrosis virus. Western blot analysis of lysates prepared from Sf9 cells infected with the recombinant viruses confirmed...