About Real-Time PCR Assays Real-time Polymerase Chain Reaction (PCR) is the ability to monitor the progress of the PCR as it occurs ( i.e., in real time). Data is therefore collected throughout the PCR process, rather than at the end of the PCR. This completely revolutionizes the way one approaches PCR-based quantitation of DNA and RNA. In real-time PCR, reactions are characterized by the point...

Rice false smut (RFS) is an important rice disease that is caused by the pathogen Ustilaginoidea virens. In this study, we developed a real-time polymerase chain reaction (PCR) assay to detect U. virens and to estimate the level of disease. The genomic DNAs of U. virens and rice were extracted together from the rice samples. Real-time PCR assays were performed and compared to conventional neste...

The disease caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) in Wuhan, China, December 2019, has rapidly spread worldwide. SARS-CoV-2 is usually detected via real-time reverse-transcription polymerase chain reaction (RT-PCR). However, the increase specimen load institutions/hospitals necessitates a simpler detection system. Here, we present an ultra-rapid, RT-PCR assay for...

Extensive detail on the application of the real-time quantitative polymerase chain reaction (QPCR) for the analysis of gene expression is provided in this unit. The protocols are designed for high-throughput, 384-well-format instruments, such as the Applied Biosystems 7900HT, but may be modified to suit any real-time PCR instrument. QPCR primer and probe design and validation are discussed, and...

background:breast cancer remains the most common and second lethal cancer in females. her-2/neu is one of the most important amplified oncogene in breast cancer. the amplification of her-2 is correlated with decreased survival, metastasis, and early recurrence.  the amplification of her-2/neu gene and synthesis of the protein are reported in 10%-34% of breast cancer cases associated with tumor ...

The identity-determining importance of nucleic acids in living beings is a guide for reaching the desired information about food quality control analyzes. With these goals, popularity Real-time PCR (Polymerase Chain Reaction) methods, which are one acid-based rapidly increasing due to their high reproducibility, precision and fast result production. It thought that informative studies analysis ...