BACKGROUND: The monocyte monolayer assay (MMA) measures the adherence and phagocytosis of sensitized red cells with peripheral monocytes. The result of MMA may reflect the in vivo destruction of sensitized red blood cells (RBCs). STUDY DESIGN AND METHODS: Blood samples from 20 patients with warm reactive autoantibodies were investigated using the MMA. The Monocyte Index (MI) values > 5% were co...

Using an immunocytochemical approach, we have demonstrated a preferential distribution of myosin isoenzymes with respect to the pattern of fiber types in skeletal muscles of the rat. In an earlier study, we had shown that fluorescein-labeled antibody against "white" myosin from the chicken pectoralis stained all the white, intermediate and about half the red fibers of the rat diaphragm, a fast-...

The purpose of this investigation was to test whether the generation of oxygen radicals is required for the cytotoxic activity of human monocytes. The cytotoxic activity of human monocytes was measured as the capacity to lyse, in vitro, human red cells sensitized with noncomplement-binding lgG anti-D alloantibodies. Lysis was determined by measuring 51Cr release from the red cells. Studies were...

The routine pretransfusion red blood cell antibody detection test (PADT), performed at the authors' institution, consists of a threephase, saline-antiglobulin technique (immediate spin, 30-minute incubation at 37 °C, IgG indirect antihuman globulin test |IAT|), and each phase is examined for hemolysis and agglutination. To determine if it would be safe to omit reading the 37 °C phase of the PAD...

Among the enzymes studied glucose-6phosphate dehydrogenase seemed the most sensitive enzyme; 57 % and 78 % inhibition at zero (due to the binding of primaquine to NADP binding site) and 30 minutes of incubation (due to the reactive oxygen species), respectively. Catalase, superoxide dismutase, and glutathione-S-transferase were less sensitive to primaquine-derived reactive oxygen species: 33, 1...

This study aims to investigate the anaerobic degradation kinetics of reactive dye, C.I. Reactive Red 141 (Evercion Red H-E7B) by partially granulated anaerobic mixed culture using three carbon sources, namely modified starch (MS), polyvinyl alcohol (PVA) and acrylic size (AS) during batch incubation. There is a first-order kinetics reaction in the decolorization processes using MS and PVA as ca...

In this paper, the effect of NaCl and Na2SO4 on the biodecolourization of reactive brilliant red K-2BP by a Halomonas sp. GYW (EF188281) was investigated in details. The decolourisation efficiency and the oxidation-reduction potential (ORP) change were explored during the decolourization process. The results from sequencing batch tests showed that Na2SO4 influenced the decolourization efficienc...

[This corrects the article DOI: 10.1371/journal.pone.0154234.].

Migrationen. Zuwanderung nach und Auswanderung aus Schwäbisch Hall 1600 – 1914 (Veröffentlichungen des Stadtarchivs Hall, Heft 33), hg. von der Geschichts-Werkstatt Red. Andreas Maisch Daniel Stihler, 2018. 404 S. mit zahlr. farb. Abb. ISBN 978-3-932146-43-5. € 25,–

This study is based on the comparative analysis of 38 patients with reactive arthritis defined by Amor criteria, a group of 33 patients with rheumatoid polyarthritis and 50 healthy controls. Antioxidant systems (glutathione peroxidase, superoxide dismutase, catalase) were investigated in inflammation consecutive to immune and infectious syndromes. Our study showed that reactive arthritis and rh...