Rapid and definitive diagnosis is critical to the prevention of the spread of endemic human pathogenic viruses. Detection of variant specific genes by reverse transcription polymerase chain reaction (RT-PCR) has become a routine diagnostic test for accurate subtyping of RNA viruses, such as influenza. In this paper, we demonstrate the use of a continuous-flow polydimethylsiloxane (PDMS) microfl...

Despite routine vaccination programs against Newcastle disease (ND), sporadic cases have occasionally occurred that remain a constant threat to commercial poultry. Ten isolates of Newcastle disease viruses (NDV) from infected broiler chicken cases were obtained from various locations in Fars province during 2009-2011 and genetically analyzed using reverse transcription polymerase chain reaction...

The diagnosis of porcine epidemic diarrhea virus (PEDV) infection in the laboratory is rather fastidious because of difficulties in virus propagation. The feasibility of virus propagation in vivo is also limited by the handling of a number of samples at the same time. In this study, the detection of PEDV by reverse transcription polymerase chain reaction (RT-PCR) is described. The RT-PCR could ...

Dengue fever is an emerging disease in Pakistan with uninterrupted increase in number of cases since 2006 Dengue fever epidemic in Lahore Pakistan. This study was planed to identify serotypes of dengue virus (DENV) involved in epidemic of 2008. The patients were screened in the microbiology laboratory with immunochromatographic kits then confirmed by antibody capture Enzyme linked immunosorbent...

Stevens-Johnson syndrome (SJS) and toxic epidermal necrolysis (TEN) are severe adverse cutaneous reactions to drugs. We describe the case of a 19 year old patient with SJS/TEN overlap syndrome, who developed severe interstitial pneumonia after she had received antiepileptic drugs. A cytomegalovirus infection was diagnosed by Real Time Polymerase Chain Reaction (RT-PCR) detection on Bronchoalveo...

genetic epidemiological studies strongly suggest that additive and interactive genes, each with small effects, mediate the genetic vulnerability for schizophrenia. with the human genome working draft at hand, candidate gene (and ultimately large-scale genome-wide) association studies are gaining renewed interest in the effort to unravel the complex genetics of schizophrenia. linkage and fine ma...

Accumulated evidence has shown that the subjective time in the sub-second range can be altered by different factors; some are related to stimulus features such as luminance contrast and spatial frequency, others are processes like perceptual grouping and contextual modulation. These findings indicate that temporal perception uses neural signals involved in non-temporal feature processes and tha...

In Niterói, state of Rio de Janeiro, dengue virus type 4 (DENV-4) was isolated for the first time in March 2011. We analysed the laboratory findings of the first cases and evaluated the use of molecular techniques for the detection of DENV-4 in Aedes aegypti that were field-caught. Conventional reverse transcriptase-polymerase chain reaction (RT-PCR) and Simplexa™ Dengue real-time RT-PCR confir...

background aggregatibacter actinomycetemcomitans and tannerella forsythensis are two major pathogens in destructive periodontal disease in humans. the detection of these bacteria is needed for diagnosis and management of the mentioned diseases. objectives we aimed to develop and compare improved multiplex conventional and sybr green real time pcr assays for a specific diagnosis of the organisms...

ABSTRACT: A statistical mechanics of neuronal interactions (SMNI) is explored as providing some substance to a physiological basis of the g factor. Some specific elements of SMNI, previously used to develop a theory of short-term memory (STM) and a model of electroencephalography (EEG) are key to providing this basis. Specifically, Hick’s Law, an observed linear relationship between reaction ti...