Dexamethasone increases the expression of adenosine A(3) receptors and augments degranulation in response to their activation in the rat basophilic leukemia cell line, RBL-2H3. We have studied the effects of dexamethasone on mast cell activation induced by A(3) receptor stimulation in vivo. Administration of the A(3) receptor agonist APNEA [N(6)-2-(4 aminophenyl)ethyladenosine; 10-30 microg kg(...

Phospholipase D (PLD) hydrolyses phosphatidylcholine to produce phosphatidic acid (PA) and choline. It has two isoforms, PLD1 and PLD2, which are differentially expressed depending on the cell type. In mast cells it plays an important role in signal transduction. The aim of the present study was to clarify the role of PLD2 in the secretory pathway. RBL-2H3 cells, a mast cell line, transfected t...

The glucocorticoid dexamethasone suppresses antigen-induced degranulation, cytokine production, and intermediate signaling events in RBL-2H3 mast cells, although the exact mechanisms are uncertain. By microarray analysis, we discovered that expression of the inhibitory adaptor protein, downstream of tyrosine kinase (Dok)-1, was up-regulated 4-fold in dexamethasone-treated RBL-2H3 cells. The up-...

Despite the high prevalence of chronic dermatitis and accompanied intractable itch, therapeutics that specifically target itching have low efficacy. Although toll-like receptors (TLRs) are suggested to contribute immune activation neural sensitization, their roles in itch skin remain elusive. Here, we show RBL-2H3 mast cell line expresses TLR4 treatment with a antagonist opposes LPS-dependent i...

Abstract Objectives Parvalbumin (PV) is the primary allergen found in fish and highly conserved. According to some studies, patients with allergy are allergic only one species of but tolerant others; however, underlying mechanism has not been identified. Materials Methods The cross-reactivity these seven parvalbumins based on turbot PV-treated mice was determined using BALB/c mouse RBL-2H3 cell...