نتایج جستجو برای: rapd و pcr

تعداد نتایج: 928078  

Journal: :Ege Journal of Fisheries and Aquatic Sciences 2017

ژورنال: :مجله دانشگاه علوم پزشکی مازندران 0
حمزه اسدی hamzeh asadi msc in microbiology, faculty of sciences, islamic azad university, qom branch, qom, iranبروجرد- دانشگاه آزاد اسلامی رضا یاری reza yari assistant professor, department of biology, faculty of sciences, islamic azad university, borujerd branch, borujerd, iranاستادیار، گروه زیست شناسی، دانشکده علوم، دانشگاه آزاد اسلامی، واحد بروجرد، بروجرد، ایران محسن زرگر mohsen zargar msc in microbiology, faculty of sciences, islamic azad university, qom branch, qom, iranکارشناس ارشد میکروبیولوژی، دانشکده علوم، دانشگاه آزاد اسلامی واحد قم، قم، ایران

سابقه و هدف: راه های تشخیص باکتری e. coli، کشت، روش های سرولوژی و ملکولی می باشد. در روش مولکولی با استفاده از پرایمرهای کوچک تصادفی، اقدام به تولید محصولات pcr می گردد. هدف از مطالعه حاضر، بررسی تنوع ژنتیکی در باکتری های اشریشیاکلی می باشد. هم چنین با استفاده از دو سویه شایع enteropathogenic e. coli (st.1) و enterohaemorrhagic e. coli (st.2)، میزان قرابت باکتری های جداسازی شده با این دو سویه ن...

Journal: :iranian journal of public health 0
sr naddaf dezfouli ma oshaghi h vatandoost e djavadian z telmadarei m assmar

anopheles fluviatilis complex is known to be a vector of malaria in iran. since mosquitoes of this species cover a wide geographical range in iran, they might have evolved into different separated populations. random amplified polymorphic dna polymerase chain reaction (rapd-pcr) assay was used to differentiate geographic populations of this species. dna was extracted from individual mosquitoes ...

Journal: :Journal of clinical microbiology 2001
A C Carvalho G M Ruiz-Palacios P Ramos-Cervantes L E Cervantes X Jiang L K Pickering

Campylobacter jejuni is one of the most common causes of bacterial diarrhea worldwide and is the primary bacterial cause of food-borne illness. Adherence to and invasion of epithelial cells are the most important pathogenic mechanisms of Campylobacter diarrhea. Molecular characterization of invasive and noninvasive Campylobacter isolates from children with diarrhea and symptom-free children was...

Journal: :Genetics and molecular research : GMR 2012
X Sun Q Mu D Jiang C Wang X C Wang J G Fang

We optimized RAPD techniques by increasing the length of RAPD primers and performing a strict screening of PCR annealing temperature to distinguish 60 sweet orange cultivars from the Research Institute of Pomology at the Chinese Academy of Agricultural Sciences. A new approach called cultivar identification diagram (CID) was used to improve the efficiency of RAPD markers for cultivar ident...

2012
Anna Lenart

The presence of Azotobacter sp. in soils has beneficial effects on plants, but the abundance of these bacteria is related to many factors, especially soil pH and fertility. The presented study evaluated the abundance of Azotobacter sp. in various soils of southern Poland and confirmed the relationship between soil properties and the presence of these bacteria. Diagnostic tests indicate that all...

Journal: :iranian journal of veterinary research 2011
r. daie ghazvini h. mirhendi s. a. ghiasian a. masoudi-nejad h. shokri

fusarium verticillioides (f. verticillioides) is not only a primary pathogen of maize, but also can causedisease in other crops such as sorghum. pathogenicity is related to mycotoxin production such as fumonisin.in the present study, 24 isolates of f. verticillioides, which were previously identified by phenotype basedmethods, were re-identified using restriction fragment length polymorphism (r...

Journal: :Entomology and applied science letters 2022

Cassia angustifolia Vahl. is native to Saudi Arabia. This shrub has been used as a laxative and even fungicide. The current study was designed evaluate the morphology molecular characterization of plants from two areas in Purified genomic DNA subjected PCR amplification using random primers (RAPD). showed that, all morphological parameters were statistically significant (P ≤ 0.05) when compa...

2011
M. Mohammadzadeh Moghaddam M. Darijani

Magnetic data is useful in both hydrocarbon exploration and mining exploration. In hydrocarbon exploration, aeromagnetic data is an effective method for locating strike-slip faulting in magnetic basement. Also, high resolution surveys enhance delineation of both subtle basement features and intrasedimentary sources. In fact, magnetic methods has evolved from its sole use for mapping basement st...

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