We examined genetic differences of four Vitis vinifera populations (A, B, C, D) including local Kabarcik cultivar grown along an altitude gradient of 800, 900, 1000, and 1150 m above sea level in the Coruh Valley (800 m: A population; 900 m: B population; 1000 m: C population; 1150 m: D population). Leaf samples were used for both RAPD and fatty acid analysis. A total of 60 individuals wi...

Using the random amplified polymorphic DNA (RAPD) technique and exploiting the unique genetics of Tetrahymena thermophila, we have identified and characterized 40 DNA polymorphisms occurring between two inbred strains (B and C3) of this ciliated protozoan. These RAPD markers permit the PCR amplification of a DNA species using template DNA from SB1969 (B strain) but fail to do so using DNA from ...

Random amplified polymorphic DNA (RAPD) was used to study the genetic variations among strains from Fusarium sambucinum isolated from wheat in Upper Egypt. Using two different primer (V6 and M13), the used strains showed a high degree of similarities and a distinct RAPD patterns. The dendrogram that constructed based on M13 primers showed that, there is no correlation between isolation sources ...

Tissue culture is a potent means for producing clonally propagated plants. However, genetically identical regenerants are of great concern due to their economic consequences. Therefore, polymerase chain reaction (PCR)-based molecular markers employed detect somaclonal variations. In this study, the genetic fidelity in vitro raised Philodendron bipinnatifidum clones, and mother plant was tested ...

A large number of micropropagated plantlets of banana, Musa acuminata var. Nanjanagudu Rasabale (NR), that were developed from axillary shoot bud explants over 10 years ago were screened for genetic variation, if any, using RAPD (Random Amplified Polymorphic DNA) and ISSR (Inter-Simple Sequence Repeats) markers. Of the 4000 in vitro plantlets, 11 were used for screening that involved shoot cult...

The usefulness of random amplified polymorphic DNA (RAPD) was examined as a potential tool to differentiate cryptic mosquito species. It proved to be a quick, effective means of finding genetic markers to separate two laboratory populations of morphologically indistinguishable African malaria vectors, Anopheles gambiae and An. arabiensis. In an initial screening of fifty-seven RAPD primers, 377...

Genetic similarity and polymorphisms among the three Egyptian water buffalo flocks (Beheiry, Minoufy and Saidy) were studied using both random amplified polymorphic DNA (RAPD) and restriction fragment length polymorphism (RFLP) techniques. Ten random primers were used to amplify DNA fragments in these three flocks. RAPD patterns with a level of polymorphism were detected among flocks. The resul...

OBJECTIVES To determine rates of carriage of fluoroquinolone-resistant Escherichia coli and extraintestinal pathogenic E. coli (ExPEC) among dogs in a specialist referral hospital and to examine the population structure of the isolates. METHODS Fluoroquinolone-resistant faecal E. coli isolates (n = 232, from 23 of 123 dogs) recovered from hospitalized dogs in a veterinary referral centre in S...

The aim of the present work is to study the antibiotic resistance, plasmid and RAPD profiles of the multidrug resistant (MDR) Coliform bacteria isolated from raw and treated sewage of Ghaziabad city, India. The MDR bacterial population in the raw and treated sewage constituted 7.5% and 19.1% of the total Coliform bacteria respectively. Five MDR Coliform bacteria (2 from raw and 3 from treated s...