Rheumatoid factor (RF) is a major source of interference in many immunoassays. Most immunoassays use mammalian polyclonal or monoclonal antibodies, and RF can react with IgG from mammalian species, thus causing false-positive results. In this work we have studied RF interference in a sandwich ELISA, where RF in the sample may react with both the capture antibody and the detection antibody to gi...

Although nanosized urinary extracellular vesicles (uEVs) are increasingly used for biomarker discovery, their isolation currently relies on time-consuming techniques hindering high-throughput application. To navigate this problem, we designed an immunoassay to isolate, quantify, and normalize uEV proteins. The uEV immunoassay consists of a biotinylated CD9 antibody to isolate uEVs, an antibody ...

Dr. Beatriz L. Gomez and colleagues described a new inhibition immunoassay for the diagnosis of histoplasmosis (1). My laboratory developed and extensively evaluated an antibody sandwich immunoassay for this purpose. It is important to contrast the two methods, as their performance characteristics differ. The characteristics of the assay developed in my laboratory are high sensitivity in dissem...

Topiramate is a newer anticonvulsant used to treat epilepsy, migraines, bipolar disorder, posttraumatic stress, and other conditions. Serum topiramate concentrations are measured to determine optimal levels, address therapeutic failure or drug–drug interactions, and assess compliance. Two high-throughput assays for serum topiramate measurement were compared: the Seradyn fluorescence polarizatio...

An automated fluorescence polarization immunoassay for the determination of vancomycin levels in serum was evaluated. The vancomycin assay is a homogeneous competitive inhibition immunoassay based on changes in fluorescence polarization that occur with antibody binding. This assay was compared with a liquid chromatographic assay and an agar well diffusion bioassay method by using clinical serum...

The rate-limiting step in many solid-phase immunoassays is associated with the slow kinetics of binding of macro-molecular antigen and conjugate to the immobilized phase. We demonstrate that the use of ultrasonic energy to enhance mass transport across liquid/solid interfaces can dramatically accelerate antigen binding to immobilized antibodies. We use an ultrasound-accelerated procedure with a...

A novel rolling circle amplification (RCA) immunoassay based on DNA enriching magnetic nanoparticles and assembled fluorescent DNA nanotags, magnetic nanoparticles-RCA immunoassay, is developed as a versatile fluorescence assay platform for highly sensitive proteins detection.

Abstract Background The high quality of antibody (Ab) is critical for an immunoassay; usually, Ab with low affinity often regarded as a “bad” one in the immunoassay development. How to use develop highly sensitive still huge challenge. Methods In this study, heterologous strategy was designed enhance sensitivity detection banned dye, rhodamine B (RB), fraudulent food. RB could not recognize by ...

background: to determine the colon transit time (ctt) in patients complaining of functional constipation and to evaluate the correlation between abnormal transit times and the types of constipation and the correlation with age and sex. materials and methods: the signs and symptoms of 84 patients with infrequent or difficult defecation, 33 males and 51 females, with a median age of 33.7 were rec...

A novel and highly sensitive immunoassay method based on surface enhanced Raman spectroscopy (SERS) and magnetic particles has been developed. This method exhibits great potential application in bio-separation and immunoassay.