Since vitamin B6 is essential for normal functioning of the central nervous system, there is growing need for sensitive analysis of B6 vitamers in cerebrospinal fluid (CSF). This manuscript describes the development and validation of a rapid, sensitive and accurate method for quantification of the vitamin B6 vitamers pyridoxal (PL), pyridoxamine (PM), pyridoxine (PN), pyridoxic acid (PA), pyrid...

Introduction: Diabetic complications as peripheral neuropathy and delayed wound healing affect patient’s quality of life may lead to amputation. Several mechanisms are involved in mediating these including accumulation Advanced Glycation End Products (AGEs) the subsequent state inflammation which were investigated current study. Methos: A fixed size was induced STZ-diabetic rats effect diabetes...

The first naturally occurring form of vitamin B6 was isolated in 1938. It has the structure, confirmed by chemical synthesis (1939), of 3-hydroxy-4,5-bis(hydroxymethyl)-2-methylpyridine (I; R = -CHzOH). The trivial name “pyridoxine,” proposed for this compound by P. GyGrgy, came into general use as a synonym for “vitamin Be.” Two other natural compounds possessing vitamin B6 activity, detected ...

The importing of vitamin B6 by renal proximal tubular cells from the rat is facilitated and Na(+)-dependent and reflects specificity for the meta-phenolate pyridinium structure with a 5-hydroxymethyl function. This transporter can, however, accept competitively each of the natural nonphosphorylated vitamers (pyridoxine, pyridoxamine, and pyridoxal) and other B6 analogues differing only in the g...

The objectives of the investigation of the nutrition of Clostridiu,m perfringens were twofold. In the first place, the development of a chemically defined medium was prerequisite for the understanding of the factors and conditions favorable to production of t#oxins or exoenzymes. Secondly, it became evident early in the investigation that the organism presented exceptional advantages as a means...

In the current study, we investigated the inhibitory activity of pyridoxine, pyridoxal, and pyridoxamine, against various digestive enzymes such as α-glucosidases, sucrase, maltase, and glucoamylase. Inhibition of these enzymes involved in the absorption of disaccharide can improve post-prandial hyperglycemia due to a carbohydrate-based diet. Pyridoxal (4.14 mg/mL of IC50) had the highest rat i...

Tyrosine aminotransferase was highly purified from rat livers in which the enzyme had been induced by a glucocorticoid hormone. The preparation eluted from a DEAEcellulose column as a single peak gave a single precipitin line by the Ouchterlony double diffusion precipitin reaction and sedimented as a single boundary in the ultracentrifuge with an s20,W of 5.9. A molecular weight of 91,000 was d...