Though the use of mutants and specific inhibitors, polyamines (putrescine spermidine, and spermine) have been shown to be essential for normal growth and development of fungi, bacteria, green plants and mammals (1). In bacteria and plants, polyamine synthesis initially involves the production of putrescine via either the ornithine decarboxylase (ODC) or arginine ARAŞTIRMA Cilt 57, No 1, S : 19 ...

It is evident that di and polyamines are intimately related to growth processes (1-3). Actively dividing mammalian cells contain higher levels of polyamines than slowly growing or quiscent cells (4,5). Polyamine levels rise In different malignancies ((3,7). On the other hand, activity of Diamine oxidase (DAO; EC 1.4.3.6) increases in human carcinomatous growths as well as in experimental neopla...

This simple, rapid liquid-chromatographic assay of urinary polyamines (putrescine, spermidine, spermine, and cadaverine) involves electrochemical detection with a post-column immobilized enzyme, polyamine oxidase (EC 1.4.3.6) from soybean seedlings. Polyamines are separated by isocratic ion-pairing reversed-phase chromatography, then enzymatically converted, with release of hydrogen peroxide, v...

this study was carried out to investigate the effects of dietary putrescine (put) on broiler’s response fed low crude protein (cp) diets. a total of 192 male day old chicks were fed with four dietary treatments including two levels of put (0 and 0.03%) and two levels of cp (normal and low) with factorial combinations. weekly growth performance, nutrient digestibility and intestinal morphology (...

Ameliorating effects of exogenous paclobutrazol and putrescine on mung bean [Vigna radiata (L.) Wilczek] under water deficit stress | Esmaeil Babarashi, Asad Rokhzadi, Babak Pasari, Khosro Mohammadi Agricultural Journals

In cultured mammary explants from both virgin and midpregnant mice which contained a low and a high initial level of putrescine, respectively, addition of an ornithine decarboxylase inhibitor, DL-a-hydrazino-Saminovaleric acid at the onset of culture prevented an early, “osmolarity-sensitive” rise in the putrescine level that occurred prior to induction of DNA synthesis by insulin. In the pres...