Culture medium used in antibody purification contains growth factors, but is usually discarded after antibody purification. We examined to determine whether the growth factors can be (i) recovered and (ii) used in subsequent cell culture. Using this medium enhanced the survival of hybridoma cells and improved antibody production. Culture medium previously used in antibody purification offers an...

We achieved the synthesis of important medronic acid monoalkyl esters via the dealkylation of mixed trimethyl monoalkyl esters of medronic acid. Two methods were developed for the purification of medronic acid monoesters: 1) small scale (10-20 mg) purification by using hydroxyapatite and 2) large scale (tested up to 140 mg) purification by high-performance countercurrent chromatography (HPCCC).

in this paper, the optimal adaptive leader-follower consensus of linear continuous time multi-agent systems is considered. the error dynamics of each player depends on its neighbors’ information. detailed analysis of online optimal leader-follower consensus under known and unknown dynamics is presented. the introduced reinforcement learning-based algorithms learn online the approximate solution...

BACKGROUND p-aminobenzamidine (p-ABA) is used as a ligand in the purification of many serine proteases and in their removal from heterogeneous samples. Moreover, p-ABA has a potent ability to bind Ca(2+)-binding proteins. The binding ability and use of p-ABA in purification processes is still not fully understood. METHODOLOGY/PRINCIPAL FINDINGS A p-Aminobenzamidine (p-ABA) ligand enabled the ...

We have partially purified human estrogen receptor from MCF-7 breast cancer cells in order to further characterize the chemical and biological properties of the receptor and to prepare specific receptor antibodies for radioimmunoassay. The estrogen receptor from MCF-7 cell cytosol was purified 1,166-fold (27% recovery) over starting cytosol by combining ammonium sulfate precipitation, affinity ...

quadruped robots have unique capabilities for motion over uneven natural environments. this article presents a stable gait for a quadruped robot in such motions and discusses the inverse-dynamics control scheme to follow the planned gait. first, an explicit dynamics model will be developed using a novel constraint elimination method for an 18-dof quadruped robot. thereafter, an inverse-dynamics...

the determination of gyration radius is a strong research for configuration of a macromolecule. italso reflects molecular compactness shape. in this work, to characterize the behavior of theprotein, we observe quantities such as the radius of gyration and the average energy. we studiedthe changes of these factors as a function of temperature for acetylcholine receptor protein in gasphase with n...

ABSTRACT It is known that various plants have purification ability for indoor air pollutants. In this paper the purification mechanism of foliage plant was investigated by analyzing bioelectrical potential. It was first shown that the air purification ability of a plant depends on various environmental conditions, especially on light irradiation. It was then clarified that the bioelectrical pot...

Cellulose is a renewable and biodegradable natural biopolymersynthesized mainly in plants such as wood, bark, cotton fiber, green algae, some bacteria and animals. Pre-treatment processes such as alkaline purification and enzymatic purification are applied in order to create a proper sorption characteristic of regenerated cellulose fiber. Prior to the application of cellulose/cellulose derivati...