Type VI secretion systems (T6SSs) are widespread in Gram-negative bacteria, including Pseudomonas. These macromolecular machineries inject toxins directly into prokaryotic or eukaryotic prey cells. Hcp proteins are structural components of the extracellular part of this machinery. We recently reported that MFE01, an avirulent strain of Pseudomonas fluorescens, possesses at least two hcp genes, ...

Meloidogyne incognita root-knot nematode is one of the main causes tomato root damage and consequently crop production losses. Thus, in vitro conditions, number nematodes hatched eggs (%) at 4 6 days mortality (J2 stage) 8, 18, 24 h, were evaluated Petri dishes containing candidate rhizobacteria Enterobacter asburiae (BA4-19 PM3-14), Acinetobacter calcoaceticus (BM2-12), Klebsiella variicola (B...

Two bacterial species; Pseudomonas fluorescens and Pseudomonas putida and three different chemical compounds; copper sulphate (CuSO4), Indole butyric acid (IBA) and potassium chloride (KCl) were tested for their ability to induce resistance in lupine plants against wilt disease caused by Fusarium oxysporum f. sp. lupini. Treatment of seeds with the selected bacterial species and chemical compou...

Protozoan predation of bacteria can significantly affect soil microbial community composition and ecosystem functioning. Bacteria possess diverse defense strategies to resist or evade protozoan predation. For soil-dwelling Pseudomonas species, several secondary metabolites were proposed to provide protection against different protozoan genera. By combining whole-genome transcriptome analyses wi...

Phosphorus deficiency is a major constraint to crop production due to rapid binding of the applied phosphorus into fixed forms not available to the plants. This work was carried out to explain the release of soluble phosphates and its effect on plant growth as a function of phosphate solubilization by fluorescent Pseudomonas. In the current study, Pseudomonas fluorescens K 34 solubilized trical...

In this study we observed the proliferation of Pseudomonas fluorescens (P. fluorescens) in mouse organ homogenates at 4 degrees C. P. fluorescens secreted a protease possessing properties different from those of the mammalian tissue proteases. The specificity of this protease required a basic amino acid residue at the P1 position at a pH optimum of 6.0. The specificity of the protease was simil...

The wild-type strain of Pseudomonas fluorescens was found to utilize a range of structurally diverse organophosphonates as its sole carbon or nitrogen sources. Representative compounds included aminoalkylphosphonates, hydroxyalkylphosphonates, oxoalkylphosphonates, and phosphono dipeptides. Among them, amino(phenyl)methylphosphonate,2-aminoethylphosphonate, aminomethylphosphonate, diisopropyl 9...

Surfaces with nanophase compared to conventional (or nanometer smooth) topographies are known to have different properties of area, charge, and reactivity. Previously published research indicates that the attachment of certain bacteria (such as Pseudomonas fluorescens 5RL) is higher on surfaces with nanophase compared to conventional topographies, however, their effect on bacterial metabolism i...

Targeted gene inactivation and metabolic profiling revealed that the cryptic PKS-NRPS gene cluster in the genome of the plant commensal Pseudomonas fluorescens Pf-5 codes for the biosynthesis of antiproliferative and antifungal rhizoxin derivatives.