Abstract Background PROTEIN PHOSPHATASE 2A (PP2A) expression is crucial for the symbiotic association between plants and various microbes, knowledge on these processes important sustainable agriculture. Here we tested hypothesis that PP2A regulatory subunits, especially B’φ B’θ, are involved in signalling mycorrhizal fungi or plant-growth promoting bacteria. Results Treatment of tomato ( Solanu...

During the last 35 years of studies of Azospirillum–plant interaction, over 20 proposals were suggested for the mechanism of action by which Azospirillum spp., the most intensively studied plant growth-promoting bacteria, enhances plant growth. The proposals include a single phytohormone activity, multiple phytohormones, nitrogen fixation, assortments of small-sized molecules and enzymes, enhan...

The association between nitrogen-fixing bacteria from the genus Azospirillum and the grasses Zea mays and Setaria italica was investigated in sterilized Leonard-jar assemblies. Nitrogen-fixing bacteria isolated from Cynodon dactylon roots in Israel and Azospirillum brasilense (Sp-7, Sp-80, and Cd) were examined. C(2)H(2) reduction activity was detected in systems containing 0.0 to 0.08 but not ...

bacteria of the genus azospirillum are well known as plant growth promoting rhizobacteria. the ability to synthesize phytohormones is considered one of the most important mechanisms to promote plant growth and is widely distributed among plant-associated rhizobacteria.the most important phytohormone produced by azospirillum is the auxin indole-3-acetic acid, with the l-tryptophan as the precurs...

Azospirillum brasilense is a nitrogen-fixing bacterium living in association with plant roots. The genome of the strain Sp245, isolated in Brazil from wheat roots, consists of one chromosome and six plasmids. In this work, the A. brasilense Sp245 plasmids were analyzed in order to shed some light on the evolutionary pathways they followed over time. To this purpose, a similarity network approac...

Revegetation on moving sand dunes is a widely used approach for restoring the degraded sandy land in northeastern China. The development of sand-fixation forest might improve structures soil microbial communities and affect N cycle. In present study, diversities nitrite (nirS nirK) nitrous oxide (nosZ) reductase genes were investigated under chronosequence Caragana microphylla shrub (9- 19-year...

Film-coating of seeds is a widely accepted technology in agriculture for effectively protecting germinating and seedlings against biotic abiotic stresses successfully delivering agro-chemicals, biomolecules, bio-inoculants to the rhizosphere region. The present experiments explored possibility using hydroxypropyl methylcellulose (HPMC) dextrin as film-coating agents impregnating maize seed with...

A lepidopteran toxin gene, cryIC (pSB607) from entomopathogenic Bacillus thuringiensis subsp. aizawai was introduced into nitrogen-fixing Azospirillum lipoferum by transformation. Regeneration of spheroplasts was achieved at 99% with 39% frequency of regeneration. Transformants were screened on NB kanamycin with ampicillin plates and 4 transformants were selected after ten generations. SDS-PAGE...

Azospirillum brasilense Sp7 and its ntrA (rpoN), ntrBC, and ntrC mutants have been evaluated for their capabilities of poly-3-hydroxybutyrate (PHB) accumulation in media with high and low ammonia concentrations. It was observed that the ntrBC and ntrC mutants can produce PHB in both low- and high-C/N-ratio media, while no significant PHB production was observed for the wild type or the ntrA mut...

The plant growth-promoting soil bacterium Azospirillum brasilense enhances growth of economically important crops, such as wheat, corn and rice. In order to improve plant growth, a close bacterial association with the plant roots is needed. Genes encoded on a 90-MDa plasmid, denoted pRhico plasmid, present in A. brasilense Sp7, play an important role in plant root interaction. Sequencing, annot...