in present study different protein measurement methods are evaluated on the allergenic extract of chenopodium album pollen, which was previously prepared in our laboratory and applied in skin prick testing in comparison with a commer¬cial extract. the protein content of similar amounts of these two extracts which had caused similar skin reactions were measured with different protein assays such...

objective(s):this study aims at exploring cytotoxic activity of different peptides derived from vsvg protein against mcf-7 and mda-mb-231 breast cancer cell lines and human embryonic kidney normal cell (hek 293).    materials and methods: the anticp web server was used to predict anticancer peptides. the cytotoxic activity of peptides with high score (p26, p7) and low score (p19) was examined b...

objective(s) homocysteine, hs-crp and fibrinogen are three novel cardiovascular risk factors. the aim of this study was to examine the effect of a single bout of circuit resistance exercise on homocysteine, hs-crp and fibrinogen levels in healthy and inactive men. materials and methods the subjects were randomly divided into the experimental group (n= 14) and the control group (n= 9). circuit t...

a pancreatopeptidase (elastase) digest index was devised for a rapid and accurate estimation of protein quality. this index was calculated on the basis of all the amino acids released by an in-vitro elastase digestion, acid hydrolyses of same sample and the residue of enzyme hydrolyzed. the amino acids were determined by thin-layer chromatography. samples used were cooked white kidneybeans, coo...

infection with hepatitis c virus (hcv) is a worldwide problem. among hcv proteins, core antigen (ag), besides its importance for diagnostic application is a prime candidate for component of a vaccine. herein, we report results of studies on production of the hydrophilic domain of core ag (2-122) in native conformation by an arabinose induction system in e.coli and the primary characterization o...

background genetic manipulation of chloroplast in higher plants offers a number of unique prerogatives, including; undesirable of pleiotropic genome and gene silencing effects and also use as an important agronomic trait for producing essential biomaterials and industrial enzymes. in order to manipulate chloroplast genome, specific vectors are required. these vectors can be transformed and expr...

the binding of drugs by plasma proteins is an important phenomenon, because it influences the size of the free fraction of the drugs in plasma. in this study the influence of dipyridamole (dp) on the protein binding of propranolol (pl) and pl on the protein binding of dp were studied alone and in the presence of nicotine (nc).        the equilibrium dialysis was employed for protein binding stu...

A sweet water-soluble protein that reminds stable over wide ranges of temperature and pH, Brazzein has various applications. Its tastes like cane sugar but have no calories. However, the extraction of brazzein from its natural source is expensive and not applicable. In this study we used recombinant DNA technology to provide an alternative option for cheaper mass production of brazzein. A brazz...

in protein biochemistry, biochemists specially study proteins as complex polymers. protein biochemistry is the study of the chemical structure of proteins, including individual amino acids, their linkage as well as physical structure of proteins. proteins, especially globular proteins, are three dimensional in nature. proper function of globular proteins is tightly related to their three-dimens...

bone mass loss is one of the commonest menopause symptoms, resulting from cessation of estrogen production. compounds which have estrogen – like biological activity similar to “isoflavones” present in plants especially soy, may reduce bone loss in postmenopausal women, because as they are similar in structure to estrogens. this study, therefore, was undertaken to assess the effect of soy protei...