نتایج جستجو برای: protamine damage
تعداد نتایج: 228243 فیلتر نتایج به سال:
side effects of ciprofloxacin (cpfx), a widely used broad spectrum antibiotic with fluoroquinolone core, have been reported in different organs. in the present study we sought to elucidate the impact of ciprofloxacin on sperm chromatin integrity and sperm dna damage using aniline blue and acridine orange technique, respectively. the fertility potential in male mice was also evaluated. nmri male...
The article by Viaro et al1 (September 2002) provides important and interesting data on the side effect profile of protamine. However, their article raises a number of important points that need addressing. First, clinically, when protamine is administered slowly, the vast majority of patients experience a minimal cardiovascular effect. A few individuals show elevated pulmonary artery pressures...
Background: Cysteine has been used as additive in cryopreservation extenders. It contains of thiol group which plays an antioxidant role to eliminate reactive oxygen species (ROS) during freeze -thaw procedure. Excessive ROS production cause DNA damage and subsequently reduce sperm fertility. On the other hand, protamine protein have high levels of cysteine which is critical in packaging and co...
Background and Objective: Protamine deficiency and DNA fragmentation have a profound effect on embryo gene expression. Incubation of sperm in culture medium leads to morphological changes of the nucleus and DNA release. However, the relationship between protamine deficiency and DNA fragments released from incubated sperm and the presence of the DNMT1 gene is not known. Materials and Methods: Se...
Key ConceptsThe two-dimensional Two-Tailed Comet assay (TT-comet) protocol is a valuable technique to differentiate between single-stranded (SSBs) and double-stranded DNA breaks (DSBs) on the same sperm cell.Protein lysis inherent with the TT-comet protocol accounts for differences in sperm protamine composition at a species-specific level to produce reliable visualization of sperm DNA damage.A...
Several gene expressions are related to the success of spermatogenesis. Protamine plays an important role in sperm DNA packaging, spermatogenesis, and quality. This study aimed isolate RNA from spermatozoa determine expression profiles protamine 1 (PRM1) 2 (PRM2). Six Pasundan bulls aged 5-8 years with a body weight 380 kg-430 kg were used for this study. The classified into group A (70%-79%) B...
introduction: pronuclear size, morphology and orientation are known to affect embryo quality and implantation. the present study was aimed at evaluation of the possible relationship between protamine deficiency with pronuclear morphology and also cleavage and embryo quality. material and methods: semen sample of 160 candidate icsi and ivf couples were analyzed for sperm concentration, and motil...
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