Superoxide dismutase (SOD), total antioxidant capacity (TAC), and thiobarbituric acid reactive substances (TBARS) in seminal plasma were evaluated on the basis of receiver operating characteristics (ROC) analysis as predictors for distinguishing satisfactory from unsatisfactory boar semen samples after storage. SOD on day 0 correlated significantly with progressive motility (r=-0.686; P<0.05) a...

Oxidative stress, resulting from excessive levels of ROS in semen, has a negative impact on functional parameters and sperm fertility. In this study we examined the influence of the oxidative stress induced by hydrogen peroxide (H2O2) in ovine sperm motility after thawing and catalase (CAT) ability to preserve sperm motility. Semen was incubated at 37°C with 100 μM H2O2, 1 U catalase + 100 μM H...

Aim of this study is to evaluate the effect adding different levels Zinc and Selenium on extender as antioxidants improve Friesian-bull semen quality after cryopreservation. Semen was collected, pooled, extended with five treatment extenders, including control without (E1), 0.3 mg/100ml Zn (E2), 0.6 (E3), 0.2 Se (E4), 0.5 (E5). evaluated for visual parameters dilution, equilibration, thawing. A...

The aim of the present study was to evaluate cryoprotective effect trehalose on stallion semen quality like progressive sperm motility, viability, acrosome integrity, membrane integrity. A total 42 ejaculates from six Marwari (seven ejaculate each) were used. Immediately after gross evaluation, filtered into a pre warm, graduated measuring bottle get gel free and it diluted with primary extende...

BACKGROUND Reverse pharmacology for drug development has been highly productive and cost-effective in recent past as it is based on the documented therapeutic effects of plants in ancient texts. Afrodet Plus(®) is formulated for the treatment of male infertility, which contains ancient herbo-minerals. Its efficacy and safety are validated through this animal study in reverse pharmacology mode. ...

Background: The application of laparoscopic method for intrauterine insemination is limited in sheep industry by the high cost of equipment and low pregnancy rate of frozen-thawed spermatozoa. Both disadvantages could be decreased if chilled ram semen is transported to the farms and inseminated through cervix. Semen extender provides the requirements for sperm and affects its motility, viabilit...

The present study was conducted to investigate the effects of butylated hydroxytoluene (BHT) supplementation on diluted, cooled and frozen-thawed ram spermatozoa. After primary evaluation of collected ejaculates, only semen samples with motility of more than 70% and sperm concentration higher than 3×10 sperm/ml were used for cryopreservation. The selected semen samples were then pooled and dilu...

Ram seminal plasma increases the fertility of frozen-thawed ram spermatozoa deposited into the cervix. The aim of the current study was to compare the effect of ram seminal plasma to that of bull seminal plasma, dog prostatic fluid, protein-free TALP TrilEq (Triladyl with 0.5 mt of Equex STM paste added to each 100 mt) and heat-treated skim milk on longevity and percentages of progressively mot...

Two experiments were designed to evaluate the effects of the amino acids glycine and cysteine on cryopreservation of ram spermatozoa. After primary evaluation of collected ejaculates, the semen samples were pooled and diluted 1:4 before cooling (experiment 1) and freezing (experiment 2) with Tris-Citrate-Fructose-Yolk (TCFY) extender supplemented with different concentrations of glycine and cys...

Background: Interleukin-17 (IL-17) is a proinflammatory cytokine that involved in the pathogenesis of several autoimmune and inflammatory diseases. Inflammation testis male-reproductive tracts may lead to male infertility.Objective: To assess seminal plasma IL-17 infertile patients evaluate its relation different parameters.Patients Methods: Fifty with primary infertility were included addition...