A cell suspension was prepared from immature rat ovaries by treatment with trypsin and collagenase. The isolated cells were capable of converting [8-14-C]adenine to cyclic [-14-C]AMP and the rate of this conversion was stimulated in vitro by luteinizing hormone and human chorionic gonadotropine, but not by prolactin, norepinephrine, dopamine or albumin. The accumulation of progesterone was al...

BACKGROUND It has been previously suggested that angiogenesis occurs during the menstrual cycle. Moreover, a rise in uterine blood flow is largely maintained by vasodilatation and substantial increases in angiogenesis. It is known that estradiol (E2) and progesterone (P4) are involved in angiogenesis. Recently, endothelial progenitor cells (EPCs) were found to be involved in neovascularization;...

Monoamine oxidase activity was measured in the brains and uteri of neonatal rats exposed to preand/or postnatal progesterone. Daily doses of progesterone were injected subcutaneously into pregnant females on gestational days 8-17 and postpartum days 2-6. On postpartum day 1, half of the pups of each litter were crossfostered to a female in the opposite experimental condition. Pups were sacrific...

The aim of study was to evaluate endocrine changes in PCOS women during metformin treatment. One hundred women with PCOS, aged 20-40 years were included. A complete hormonal and metabolic pattern was recorded for each subject every 6 months. Metformin treatment after 6 and 12 months significantly reduced weight, BMI, waist circumference, insulin and HOMA-IR (p=0.000) with high differences of va...

Avian eggs contain a variety of steroid hormones, which have been attributed as a tool for maternal phenotypic engineering. The majority of studies focuses on androgens, but also significant amounts of progesterone as well as other steroid hormones have been measured. The question if corticosterone is also present in eggs of chickens is currently under debate. The only analytical validation per...

The effect of prostaglandin F2α (PGF) treatment during development of the corpus luteum on concentrations of plasma progesterone, LH, and FSH and the resulting temporal interrelationships among hormones were studied in 72 mares in two experiments. In experiment 1, a single treatment on Day 0 (day of ovulation) or Day 1 was associated with a significant increase in progesterone, but the increase...

Plasma progesterone levels during the follicular and luteal phases were compared when measured simultaneously by competitive protein binding (using guinea pig sera as binding agent) and radioimmunoassay (antiserum against progesterone-11-alpha-succinyl bovine serum albumin in rabbits), the values obtained were significantly different within each technique, depending on whether previous thin-lay...

One of the most remarkable immunological regulations is the maternal immune tolerance toward the fetal semiallograft during pregnancy, which has been referred to as immunity's pregnant pause. Rejection of the semiallogeneic trophoblast cells must be selectively inhibited and pathways presumably include Th2 cytokines unopposed by Th1 cytokines. Steroid hormones, including progesterone, have simi...

In the study, 1118 Polish Black and White cows from a dairy herd were examined during a three-year period. In 388 (34.7%) animals no visible oestrus signs were observed until day 60 postpartum. In those cows clinical examinations were performed twice, with a 10 d interval, and blood samples for progesterone analysis were collected simultaneously. Progesterone was measured by RIA. Based on clini...

Objective: A significant percent of survivors from cardiac arrest and resuscitation suffer permanent brain damage. Previous studies have demonstrated the Neuroprotective effects of progesterone on regional brain ischemia and injury. In the present study, we investigate the Neuroprotective effects of progesterone on global brain ischemia as a result of cardiac arrest and resuscitation. Subjects:...