We report that freshly isolated, unstimulated Kupffer cells (KC) from MRL/lpr female mice in short-term culture spontaneously produce high levels of TNF-alpha. TNF production was first detected in KC cultures at age 6 wk and increased with the age of the mice. Moreover, the levels of spontaneous TNF production by KC directly correlated with the age of the MRL/lpr mice. Although TNF production b...

the taguchi design of experiments was used to test the relative importance of medium components and environmental factors on poly(β-hydroxybutyrate)(phb) production by ralstonia eutropha. the optimum condition was obtained as: fructose concentration, 15 g/l; c/n ratio, 7.4; agitation speed 200 rpm; culture time, 40 h; temperature, 25 ° c; seed age, 15 h. at optimum condition the yield of phb pr...

The Taguchi design of experiments was used to test the relative importance of medium components and environmental factors on poly(β-hydroxybutyrate)(PHB) production by Ralstonia eutropha. The optimum condition was obtained as: fructose concentration, 15 g/L; C/N ratio, 7.4; agitation speed 200 rpm; culture time, 40 h; temperature, 25 ° C; seed age, 15 h. At optimu...

the aim of this descriptive- correlational study was to assess trout farm fisheries owners' managerial skills and its relation with their farm yield. the statistical population consisted of all trout farm fisheries with official production license in mahneshan county (147 units) which 110 units as sample selected by multi-stage randomized sampling method based on cochran's sampling fo...

in this experiment, the influence of prebiotic and probiotic supplementation in the broiler breeder diets on body weight, mortality, feed intake, egg production, hatchability and humoral immunity response was investigated. a total number of 13140 female and 1260 male breeders (cobb 500) with 26 wks of age were allocated to three treatments with six replicates (800 birds each replicate). breeder...

An epigenetic profile defining the DNA methylation age (DNAm age) of an individual has been suggested to be a biomarker of aging, and thus possibly providing a tool for assessment of health and mortality. In this study, we estimated the DNAm age of 378 Danish twins, age 30-82 years, and furthermore included a 10-year longitudinal study of the 86 oldest-old twins (mean age of 86.1 at follow-up),...