نتایج جستجو برای: primary culture
تعداد نتایج: 897287 فیلتر نتایج به سال:
Microglia is considered the only cell population of mesodermal origin, which comprises 10% of the cells in brain parenchyma. Recent neural stem cell (NSC) studies demonstrate that the brain has regenerative potential. NSCs do not give rise to microglial cells, however indicating that NSCs alone cannot complete the regenetion of the brain. Although the role of microglia is not fully understood, ...
In the present study, the primary cell lines were derived from caudal fin explants of the Caspian Kutum (Rutilus frisii kutum), using two treatments (tissue culturing without enzyme and after trypsin treatment). For this purpose, pieces of caudal fin tissue (1 millimeter cubic) were isolated from 10 healthy young fish with an average weight of 12±1 g and a mean length of 11±1cm in sterile condi...
théoriquement, lenseignement constitue, dans la relation didactique, un pôle symétrique à celui de lapprentissage. du point de vue du curriculum, lenseignement est généralement caractérisé comme le moyen par lequel le curriculum est mis en oeuvre. les progrès dans les connaissances psychologiques sur les phénomènes dappropriation ont montré quun savoir est véritablement approprié, cest-à-d...
The primary culture of neuronal cells plays an important role in neuroscience. There has long been a need for methods enabling the long-term culture of primary neurons at low density, in defined serum-free medium. However, the lower the cell density, the more difficult it is to maintain the cells in culture. Therefore, we aimed to develop a method for long-term culture of neurons at low density...
PURPOSE Burnout is a threat to the primary care workforce. We investigated the relationship between team structure, team culture, and emotional exhaustion of clinicians and staff in primary care practices. METHODS We surveyed 231 clinicians and 280 staff members of 10 public and 6 university-run primary care clinics in San Francisco in 2012. Predictor variables included team structure, such a...
Recently, the presence of telocytes was demonstrated in human and mammalian tissues and organs (digestive and extra-digestive organs, genitourinary organs, heart, placenta, lungs, pleura, striated muscle). Noteworthy, telocytes seem to play a significant role in the normal function and regeneration of myocardium. By cultures of telocytes in two- and three-dimensional environment we aimed to stu...
many investigators have used xenogeneic, especially murine stromal cells and fetal calf serum to maintain and expand human stem cells. the proliferation and expansion of human hematopoietic stem cells in ex vivo culture were examined with the goal of generating a suitable protocol for expanding hematopoietic stem cells for patient transplantation. using primary fetal liver cells, we established...
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