Since the birth of first IVF baby, Louise Brown, in 1978, researchers and clinicians have sought ways to improve pregnancy outcomes through embryo selection. In 1990s, blastomere biopsy fluorescence situ hybridization (FISH) were developed human embryos for assessment aneuploidy translocations. Limitations number chromosomes that could be assayed with FISH lead development comparative genomic (...

Preimplantation genetic diagnosis (PGD) involves testing for specific genetic conditions prior to the implantation [5] of an embryo in the uterine wall. This form of genetic screening has been made possible by the growth of in-vitro fertilization [6] (IVF) technology, which allows for the early stages of development to occur in a laboratory dish rather than in vivo [7]. The purpose of PGD is to...

Objective: To evaluate the use of multiple displacement amplification (MDA) for whole-genome amplification in the preimplantation genetic diagnosis (PGD) of Marfan syndrome. Design: Multiple displacement amplification was used to amplify the whole-genome directly from a single cell. The MDA product was used for polymerase chain reaction (PCR) analysis of five different loci. At this point MDA w...