The number of follicles were compared in different developmental stages after the cryopreservation of human ovarian tissue by open freezing system followed by xenotransplantation into severe combined immunodeficient (SCID)-mice under stimulation, with and without ovariectomy. Ovarian tissue, cryopreserved for fertility preservation was partly examined by LIVE/DEAD viability staining or was tran...

To compare the efficiency of North Carolina State University medium 23 (NCSU23) and Alpha Minimum Essential Medium (?-MEM) as a base medium, to evaluate effects Anti-Müllerian Hormone (AMH) alone or in combination with Follicle Stimulating (FSH) on vitro development steroid production isolated porcine preantral follicles. Porcine secondary follicles were cultured NCSU23 ?-MEM media for 4 days. ...

Abstract Study question Does Mitoquinol have a protective effect on survival and mitochondrial oxidative stress in cultured human preantral follicles? Summary answer significantly increased of follicles for 8 days decreased expression genes related to the response. What is known already Culturing ovarian promising new source mature oocytes fertility preservation, as well key model system invest...

introduction: among the endocrine diseases, hypothyroidism is the commonest after diabetes.thyroid hormones (t3,t4) are essential for genital organs function. apoptosis process in ovarian cells plays a significant role of development in ovarian follicles. the aim of the present study was determine the apoptotic index induced by hypothyroidism in rat ovarian follicles. materials and methods: in ...

The present study was aimed to study the effect of an ovine follicular fluid peptide on ovarian follicle and good oocyte numbers and weights of ovary, uterus, liver, pancreas and kidney in rats, R. norvegicus. A 30.1 kDa peptide was isolated from ovine follicular fluid by ammonium sulphate precipitation and then gel filtration. The peptide was tested at various levels in normal (22 and 36 day-o...

Background The aim of this study was to investigate the effect of applying the 1-mT magnetic field (MF) in the ovarian tissue vitrification. MaterialsAndMethods Ovaries of 6-8 week-old Naval Medical Research Institute female mice randomly were divided into 5 groups: V1: Ovaries that immediately after leaving of the mice body, were examined histologically (control group), V2: Ovaries that withou...

Background: The aim of this study was to investigate the compare of Conventional (CV) and Direct Cover (DCV) vitrification and effect of sodium selenite(SS) that supplemented with FBS and BSA on reactive oxygen species (ROS) production, total antioxidant capacity (TAC) and glutathione peroxide (GPx) activity of cultured pre-antral follicles derived from vitrified and non-vitrified ovarian tissu...

Background: Nanoparticles (NPs) are small-sized particles with dimensions of 1 - 100 nm that have various medical and pharmaceutical technology applications. Objectives: In this study, we aimed to develop a quick, green, eco-friendly procedure for the synthesis gold NPs (AMGNPs) using aqueous extract Achillea millefolium (AM) evaluate effects them on preantral follicle (PF) maturation in vitro ...

Gap junction communication plays an essential role in follicle growth. Immunocytochemistry and confocal microscopy were used to examine the expression of gap junction connexins of the alpha and beta subfamilies in follicles from primordial to preovulatory stages in the ovaries of prepubertal and adult mice. Connexin-specific antibodies detected alpha(1), alpha(4), alpha(6), beta(1), beta(2) and...

The cytologic localization and cellular levels of TGF alpha in the human ovary during follicular growth and regression were examined by avidin/biotin immunoperoxidase techniques with a monoclonal antibody to TGF alpha. In primordial follicles, only the oocyte showed intense immunostaining for TGF alpha, whereas the flattened pregranulosa cells were negative for the immunostaining. The earliest ...