Xanthine oxidase (X0) has been thoroughly studied by many investigators and the range of possible substrates of this enzyme is well known (1, 2). In the early experiments, enzymatic activity was measured by decolorization of a suitable dyestuff such as methylene blue or by oxygen consumption. Therefore, the exact pathway of oxidation remained unknown for many substrates. The spectrophotometric ...

This study makes a comparison between Fenton, Fenton-like, photo-Fenton and photo-Fenton-like treatment methods to investigate the removal of two acid dyes. The two selected acid dyes are C.I. Acid Orange 8 (AO8) and C.I. Acid Red 17 (AR17). These were chosen as model organic contaminants. The effect of operating parameters, such as amount of Fenton’s reagent (H2O2 and Fe) and Fenton’s-like rea...

The gene (CcLcc2) encoding laccase from the basidiomycete Coprinopsis cinerea Okayama-7 #130 was synthesized by polymerase chain reaction-based two-step DNA synthesis, and heterologously expressed in Pichia pastoris. The recombinant protein was purified by ammonium sulphate precipitation and nickel nitrilotriacetic acid chromatography. The molecular mass of CcLcc2 was estimated to be 54 kDa by ...

In this study, pure ZnO, CeO2 and ZnO/CeO2 nanocomposites were synthesized using a thermal decomposition method and subsequently characterized using different standard techniques. High-resolution X-ray photoelectron spectroscopy measurements confirmed the oxidation states and presence of Zn(2+), Ce(4+), Ce(3+) and different bonded oxygen species in the nanocomposites. The prepared pure ZnO and ...

Three new methods are described for the assay of lamivudine in bulk drug and in tablet dosage forms using chloramine-T and two dyes, methyl orange and indigo carmine, as reagents. In titrimetry, aqueous solution of lamivudine is treated with a measured excess of chloramine-T in sulphuric acid medium and after the oxidation of lamivudine is judged to be complete, the unreacted oxidant is determi...

The oxidative degradation of Procion Red MX-5B by solar Fenton’s has been investigated using sunlight as irradiation source in presence of hydrogen peroxide and Fe2+. Maximum decolorization (99%) and COD removal (97%) for MX-5B was obtained at H2O2 dose of 16.6 mM and at 30 min irradiation time. Fe2+ dose of 0.36 mM was optimum for Procion Red MX-5B degradation. The solar degradation mechanism ...

Xanthine oxidase (X0) has been thoroughly studied by many investigators and the range of possible substrates of this enzyme is well known (1, 2). In the early experiments, enzymatic activity was measured by decolorization of a suitable dyestuff such as methylene blue or by oxygen consumption. Therefore, the exact pathway of oxidation remained unknown for many substrates. The spectrophotometric ...

A series of long-chain methyl esters with vicinal oxo groups (1,2-diones; 1,2-diketones) were synthesized by potassium permanganate-based oxidation of methyl esters of mono-unsaturated fatty acids. The presence of two additional carbonyl groups may facilitate the synthesis of other derivatives. The starting materials were selected in such a fashion to give the 1,2-dioxo moiety in consecutive po...