background: vasopressin type 2 receptor (v2r) plays an important role in the water reabsorption in the kidney collecting ducts. v2r is a g protein coupled receptor (gpcr) and the triplet of amino acids aspartate-arginine-histidine (drh) in this receptor might significantly influence its activity similar to other gpcr. however, the role of this motif has not been fully confirmed. therefore, the ...

results no borrelia dna was detected by pcr in skin lesions of patients with morphea. patients and methods sixty six patients with morphea were prospectively included in the present study. for each patient, formalin-fixed, paraffin-embedded tissue blocks of skin lesion biopsies were examined for borrelia spp. dna using pcr. objectives we aimed to find the frequency of borrelia in morphea lesion...

conclusions this study suggests that the vaca pcr can be very useful in diagnosis, treatment, and follow-up of patients with gastritis and pud. the pcr assay and clo test can complement and confirm each other in identification and eradication of cancer-related strains of h. pylori. the exclusion of patients with suspected achlorhydria from the study is likely a reason for the lack of false posi...

streptococcus agalactiae is acommensalorganism, but it may cause infection in susceptible hosts. the aim of this study was to evaluate pcr assay compared with conventional culture method for direct detection of streptococcus agalactiae . total of 203 paired low vaginal swabs were collected from women at 35-37 weeks of pregnancy from june 2013 through february 2014 for detection of streptococcus...

background :polymerase chain reaction (pcr) assay has widely used for the detection of tuberculosis (tb). this study tried to compare in-house pcr with some well-known commercial pcr kits for detection of tb agent. methods : clinical samples obtained from 620 tb suspected patients were analyzed for the diagnosis of mycobacterium tuberculosis complex (mtc) by in-house pcr. all samples were obtai...

background toxoplasmosis is a parasitic disease caused by the protozoan toxoplasma gondii. this parasite infects most of warm-blooded animals, including birds. turkeys are one of these animals which might be infected by this parasite. little is known about the prevalence of t. gondii in turkeys in iran. objectives the current study aimed to evaluate the rate of toxoplasma infection in turkeys i...

Abbreviations: DMS: Dimethyl Sulphate; ds: Double-Strand; HCV: Hepatitis C Virus; NMIA: N-Methylisatoic Anhydride; NS: Nonstructural; ORF: Open Reading Frame; RT-qPCR: Reverse Transcription-Quantitative Polymerase Chain Reaction; SD: Standard Deviation; SEM: Standard Error of the Mean; SL: Stem-Loop; ss: SingleStrand; SHAPE: Selective 2'-hydroxyl Acylation Analyzed by Primer Extension; UTR: Unt...