conclusions results of the current study confirmed that molecular methods such as pcr and lamp were much more sensitive, rapid, and specific than culturing to identify non-culturable coccoid forms of h. pylori in water. results ten copies of targeted dna were required for pcr detection whereas only five copies gave a positive reaction by lamp assay, with 100% specificity. of the 10 isolates ino...

results among the 225 isolates, 33.7% (76/225) and 18.1% (41/225) were resistant to imipenem and meropenem, respectively. of the 76 imipenem-resistant p. aeruginosa strains, 45 (59.2%) were positive for mbls, 34 (75%) strains carried the blaimp-1 gene, and 1 (2.2%) strain carried the blavim-2 gene. conclusions our results showed that there was a high frequency of imp-1 positive p. aeruginosa in...

conclusions a high rate of 25% and 24.13% of hbv dna was detected among patients with hodgkin and non-hodgkin lymphoma, respectively. results three out of 12 (25%) hodgkin samples and seven out of 29 (24.13%) non-hodgkin showed positive hbv dna results. the results of sequencing revealed that the d genotype was predominant among the positive hbv patients. interestingly an unpredictable amino ac...

conclusions human papillomavirus 16 as a high risk hpv was found to have the highest frequency among patients with anogenital warts. results out of 54 samples, 46 (85.18%) cases showed positive results for hpv dna. a total of 26 (56.6%) samples were males and 20 (43.4%) females while eight (14.81%) showed hpv negative results. overall, 37 (80%) patients had multiple sexual partners, and nine (2...

background: the emerge of rapid and accurate detection of meticillin-resistant staphylococcus aureus (mrsa) has been highlighted. objectives: the current study evaluated the prevalence of mec-a gene in biological specimens of various medical wards, in order to determine any possible relationship. patients and methods: using traditional culture methods, 250 isolates were detected. the prevalence...

conclusions: even, very low amount of csf-igg with good specificity (90%); could differentiate cases and controls (p = 0.000). the csf-igg test (sensitivity 70%, npv 28.8%) was weak for ruling out the m. pneumonia in cases. positive pcr was rare (2%) in csf of cases and controls but is more reliable for diagnosing the recent m. pneumonia infection. we prefer to assay the csf for both serology a...

abstract       background and objectives: plasmodium falciparum merozoite surface protein-1 (pfmsp-1) is a promising vaccine against malaria during its blood stages which play an important role in immunity to this disease. polymorphic nature of this gene is a major obstacle in making an effective vaccine against malaria. in this study, the genetic diversity of plasmodium falciparum isolates was...

in this study, an efficient agrobacterium-mediated transformation method was developed forpomegranate (punica granatum l.), a difficult-to-transform plant. in vitro shoot segments wereinoculated with agrobacterium tumefaciens strain lba4404 harboring the binary vectorpbi121 carrying the neomycin phosphotransferase (nptii) gene as a selectable marker and β-glucuronidase (gus) gene as a reporter....

avian adenovirus was isolated from naturally infected birds during 1990 to 2003 in the vicinity ofkarachi, pakistan. the virus is known to cause hydro-pericardium syndrome (hps) in poultry. the mortalitywas recorded 20-50% in commercial broilers. fifty field samples were collected; however, six samples wereselected for molecular studies. these isolates were grown and characterized by cell cultu...

five common tetracycline resistance genes tet(a), tet(b), tet(m), tet(o) and tet(s) were studied by polymerase chain reaction in 100 bacteria isolated from iranian fish farms. in the antibiogram test most of the bacteria were either intermediately or completely resistant to tetracycline. nine isolates out of 46 aeromonas spp. contained either tet(a/m/s) resistant genes as follows: tet(a) in a. ...